Dataset Information

Quantitative phosphoproteomics analysis of olfactory receptor signaling in prostate cancer cells

ABSTRACT: The olfactory receptor PSGR1 is not only expressed in olfactory tissue but shows expression in the prostate. In addition, the expression of this receptor is elevated in prostate cancer as well as other prostate diseases and can be used as diagnostic marker. The signalling cascade triggered by the activation of this receptor remains largely unknown until now. To investigate PSGR1 signalling, we stimulated the PSGR1 receptor with one of its known ligands, the odorant beta-ionone, and used a phosphoproteomics approach to identify mediators of PSGR1 signalling. We found the non-receptor tyrosine kinase Pyk2, the tumour suppressor NDRG1 as well as the sodium/hydrogen exchanger NHE1 to be downstream targets of activated PSGR1.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Prostate Cancer Cell Line

DISEASE(S): Prostate Adenocarcinoma

SUBMITTER: Friedel Drepper

LAB HEAD: Bettina Warscheid

PROVIDER: PXD000985 | Pride | 2016-07-08

REPOSITORIES: Pride

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Quantitative phosphoproteomics reveals the protein tyrosine kinase Pyk2 as a central effector of olfactory receptor signaling in prostate cancer cells.

Wiese Heike H Gelis Lian L Wiese Sebastian S Reichenbach Christa C Jovancevic Nikolina N Osterloh Markus M Meyer Helmut E HE Neuhaus Eva M EM Hatt Hanns H HH Radziwill Gerald G Warscheid Bettina B

Biochimica et biophysica acta 20140909 6

The prostate-specific G-protein-coupled receptor 1 (PSGR1) is an olfactory receptor specifically expressed in the prostate gland. PSGR1 expression is elevated both in benign prostatic hyperplasia tissue and in prostate cancer. Stimulation of PSGR1 by the odorant β-ionone leads to an increase in the intracellular Ca(2+) concentration, activation of mitogen-activated protein (MAP) kinases and a decrease in prostate cancer cell proliferation. To further extend our knowledge about PSGR1 signaling in ...[more]

PMID: 25219547

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