Dataset Information

Egg yolk proteins G. fossarum - High-throughput proteome dynamics for discovery of key proteins in sentinel species: Unsuspected vitellogenins diversity in the crustacean Gammarus fossarum

ABSTRACT: We aimed to specifically identify the major egg yolk proteins (i.e. vitellogenin) in the amphipod Gammarus fossarum, a sentinel species in freshwater risk assessment. Vitellogenin production in female is vital for the embryonic development and its production in male organism is commonly employed as a biomarker of exposition to xenoestrogens in fish. We performed a shotgun proteomics analysis on embryos and ovaries at different stages of their development. The proteome dynamics over the cycle was analyzed to correlate on the first hand proteins which are accumulating along vitellogenesis and on the other hand, proteins with decrease amounts long embryogenesis. This information led to the proposal of putative novel VTG candidates. Our data provides the first large scale molecular description of a crustacean reproductive cycle, while our temporal analysis evidenced seven candidates proteins as “true vitellogenin” in G. fossarum.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Gammarus Fossarum

TISSUE(S): Embryo, Oocyte

SUBMITTER: Jean ARMENGAUD

LAB HEAD: Jean Armengaud

PROVIDER: PXD001002 | Pride | 2016-07-15

REPOSITORIES: Pride

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High-throughput proteome dynamics for discovery of key proteins in sentinel species: Unsuspected vitellogenins diversity in the crustacean Gammarus fossarum.

Trapp Judith J Armengaud Jean J Gaillard Jean-Charles JC Pible Olivier O Chaumot Arnaud A Geffard Olivier O

Journal of proteomics 20160709

<h4>Unlabelled</h4>In environmental science, omics-based approaches are widely used for the identification of gene products related to stress response. However, when dealing with non-model species, functional prediction of genes is challenging. Indeed, functional predictions are often obtained by sequence similarity searches and functional data from phylogenetically distant organisms, which can lead to inaccurate predictions due to quite different evolutionary scenarios. In oviparous females, vi ...[more]

PMID: 27404005

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Project description:An important facet of the oocyte to embryo transition in mammals is the rapid elimination of a subset of the maternal products that got accumulated during oogenesis. RNA studies support a view that the transition occurs quite rapidly. Whether this applies also for proteins remains to be determined beyond the very few cases known to date. We report that COPS3, the 3rd subunit of the COP9 signalosome complex, forms a large protein deposit in mouse oocytes (94th percentile of the riBAQ distribution). The one and only knock-out study had previously shown that Cops3 null (-/-) mouse embryos obtained from heterozygous intercrosses arrested after 5.5 dpc and were resorbed by 8.5 dpc mainly due to increased cell death in the epiblast (PMID: 12972600). However, more recent studies from our group ascribed Cops3 gene with a developmental role already at the 2-cell stage. Specifically, the sister blastomeres differ from each other in Cops3 mRNA levels and distribution, preceding the discordance of epiblast formation in derivative twin blastocysts, and implicating Cops3 in the molecular underpinnings of totipotency. This discrepancy between original knock-out result and recent observations can be resolved if we posit that the first requirement for Cops3 gene function was bridged by maternal protein that outlived the locus excision, consistent with the observation that Cops3 -/- blastocysts had the same immunostaining intensity as the +/- or +/+ counterparts in the original knock-out study. Thus, these contradicting observations support a hypothesis that 5.5 dpc may not have been the first time when the gene function was needed in development, but the second time. To test this hypothesis, pronuclear-stage mouse oocytes were subjected to an immunological method that directly targets the protein of interest by way of proteasomal degradation of the COPS3-antibody-TRIM21 complex. Briefly, pronuclear-stage mouse oocytes were microinjected with a mixture of the COPS3-specific purified monoclonal IgG antibody, mCherry-Trim21 mRNA and Oregon Green dextran beads (inert tracer). Injected oocytes were then allowed to develop and collected for lysis 24 hours later, when they reached the 2-cell stage but were not able to progress further. In addition to the COPS3, we targeted two additional proteins, OCT4 and TEAD4. The following seven experimental groups were examined by liquid chromatography-mass spectrometry (LC-MS/MS) using the pipeline described previously by Israel et al.: 1) non-manipulated 2-cell embryos; 2) 2-cell embryos from oocytes injected with Oregon Green dextran beads; 3) 2-cell embryos from oocytes injected with mCherry-Trim21 mRNA and Oregon Green; 4) 2-cell embryos from oocytes injected with mCherry-Trim21 mRNA, Oregon Green and anti-COPS3 (Abcam 79698); 5) 2-cell embryos from oocytes injected with anti-COPS3 (Abcam 79698) and Oregon Green; 6) 2-cell embryos from oocytes injected with mCherry-Trim21 mRNA, Oregon Green and anti-TEAD4 (Abcam 58310); 7) 2-cell embryos from oocytes injected with mCherry-Trim21 mRNA, Oregon Green and anti-OCT4 (Abcam 181557).

			Action	DRS
	Embryo_S1-n1.RAW	Raw
	Embryo_S1-n1.mgf	Mgf
	Embryo_S1-n1.pride.mgf.gz	Mgf
	Embryo_S1-n2.RAW	Raw
	Embryo_S1-n2.mgf	Mgf