Proteomics

Dataset Information

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Identification of LMTK3 protein by LC/MS/MS Analysis of 1D SDS PAGE Gel Bands

ABSTRACT: Identification of LMTK3 by LC/MS/MS. A plasmid encoding for full-length LMTK3 was overexpressed in MCF-7 breast cancer cells. Following antibody detection by WB, two bands of interest were seen at around 200 kDa and 150 kDa on the blotting membrane. Colloidal Coomassie (CCBB) staining was performed on a separate gel, to which the Western blot was aligned with the gel markers and a region of gel corresponding to the bands of interest were excised, trypsin digested and analysed by LC/MS/MS.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Breast Cancer

SUBMITTER: Georgios Giamas  

LAB HEAD: Georgios Giamas

PROVIDER: PXD001004 | Pride | 2022-02-22

REPOSITORIES: pride

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Dataset's files

Source:
Action DRS
F033998.dat Other
F033999.dat Other
File Name_ PR329_GG2_1.raw (F033998).mzid_File_Name__PR329_GG2_1.raw_(F033998).pride.mgf.gz Mzid
File Name_ PR329_GG2_1.raw (F033998).pride.mztab.gz Mztab
File Name_ PR329_GG2_2.raw (F033999).mzid_File_Name__PR329_GG2_2.raw_(F033999).pride.mgf.gz Mzid
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Publications

Lemur tyrosine kinase 3 (LMTK3) is associated with cell proliferation and endocrine resistance in breast cancer. We found that, in cultured breast cancer cell lines, LMTK3 promotes the development of a metastatic phenotype by inducing the expression of genes encoding integrin subunits. Invasive behavior in various breast cancer cell lines positively correlated with the abundance of LMTK3. Overexpression of LMTK3 in a breast cancer cell line with low endogenous LMTK3 abundance promoted actin cyto  ...[more]

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