Proteomics

Dataset Information

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In-depth phosphoproteomic profiling of mouse microglia BV-2 cell line


ABSTRACT: We present a simple strategy (integrated Multistep enzyme digestion, Enrichment, Database search, “iMEED”) to improve coverage of the phosphoproteome from less starting material that is faster than other commonly used approaches. iMEED has generated the most comprehensive phosphoproteome of murine microglia to date, comprising 8807 unique phosphopeptides in 36 LC-MS/MS runs from 300 ug of starting material.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Microglial Cell

SUBMITTER: Dohyun Han  

LAB HEAD: Dohyun Han

PROVIDER: PXD001033 | Pride | 2014-10-15

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
bv2_phospho_1-1_1.pep.xml Pepxml
bv2_phospho_1-1_1.raw Raw
bv2_phospho_1-1_2.pep.xml Pepxml
bv2_phospho_1-1_2.raw Raw
bv2_phospho_1-1_3.pep.xml Pepxml
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Publications

Integrated approach using multistep enzyme digestion, TiO2 enrichment, and database search for in-depth phosphoproteomic profiling.

Han Dohyun D   Jin Jonghwa J   Yu Jiyoung J   Kim Kyunggon K   Kim Youngsoo Y  

Proteomics 20141008 2-3


Protein phosphorylation is a major PTM that regulates important cell signaling mechanisms. In-depth phosphoproteomic analysis provides a method of examining this complex interplay, yielding a mechanistic understanding of the cellular processes and pathogenesis of various diseases. However, the analysis of protein phosphorylation is challenging, due to the low concentration of phosphoproteins in highly complex mixtures and the high variability of phosphorylation sites. Thus, typical phosphoproteo  ...[more]

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