Dataset Information

Metaprotemic analysis of groundwaters from a coal tar-contaminated aquifer in South Glens Falls, NY: MW37, Shotgun 2

ABSTRACT: Deciphering the in situ activities of microorganisms is essential for understanding the biogeochemical processes occurring in complex environments. Here we used environmental metaproteomics to obtain information about the identity and activity of subsurface microbial populations in coal-tar-contaminated groundwater. The present study reports metaproteomic data showing high representation of Candidatus Methylomirabilis oxyfera in our study site’s subsurface microbial community. In addition, eight of the nine proteins of the n-damo pathway were identified—indicating that n-damo is an active process occurring in situ in this habitat.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Prokaryotic Environmental Samples

SUBMITTER: Buck Hanson

LAB HEAD: Eugene Lewis Madsen

PROVIDER: PXD001037 | Pride | 2022-02-22

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	Hanson_Madsen_MW37E_F391144.dat	Other
	Hanson_Madsen_MW37Ere.mgf	Mgf
	Hanson_Madsen_MW37Ere.raw	Raw

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Publications

In situ expression of nitrite-dependent anaerobic methane oxidation proteins by Candidatus Methylomirabilis oxyfera co-occurring with expressed anammox proteins in a contaminated aquifer.

Hanson Buck T BT Madsen Eugene L EL

Environmental microbiology reports 20150224 2

Deciphering the in situ activities of microorganisms is essential for understanding the biogeochemical processes occurring in complex environments. Here, we used environmental metaproteomics to obtain information about the identity of subsurface microbial populations in coal tar-contaminated groundwater and the metabolic processes they catalyze. Metaproteomic libraries (two shotgun and seven slices from one SDS-PAGE gel) were generated from replicate samples of microbial biomass. Peptide fragmen ...[more]

PMID: 25403415

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