Proteomics

Dataset Information

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A High-resolution Systems-wide Screen for Substrates of the SCFβTrCP Ubiquitin Ligase

ABSTRACT: This project demonstrates a combined bioinformatics and affinity purification-mass spectrometry (AP-MS) workflow for identifying in a systems-wide manner bone fide substrates of βTrCP

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Kidney

SUBMITTER: Teck Yew Low  

LAB HEAD: Albert J.R. Heck

PROVIDER: PXD001088 | Pride | 2015-01-06

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
OR5_120205_EV_Control_01.raw Raw
OR5_120205_EV_Control_02.raw Raw
OR5_120205_EV_Control_03.raw Raw
OR8_121030_BTRCP_IP_R474A_01.raw Raw
OR8_121030_BTRCP_IP_R474A_02.raw Raw
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Publications

A systems-wide screen identifies substrates of the SCFβTrCP ubiquitin ligase.

Low Teck Yew TY   Peng Mao M   Magliozzi Roberto R   Mohammed Shabaz S   Guardavaccaro Daniele D   Heck Albert J R AJ  

Science signaling 20141216 356

Cellular proteins are degraded by the ubiquitin-proteasome system (UPS) in a precise and timely fashion. Such precision is conferred by the high substrate specificity of ubiquitin ligases. Identification of substrates of ubiquitin ligases is crucial not only to unravel the molecular mechanisms by which the UPS controls protein degradation but also for drug discovery purposes because many established UPS substrates are implicated in disease. We developed a combined bioinformatics and affinity pur  ...[more]