Proteomics

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Human Sumoylome (SUMO-2) upon bacterial infection

ABSTRACT: Given the role of SUMOylation in pathogenic infection, we wanted to evaluate the changes to the host SUMO-2 subproteome during Shigella infection. HeLa cells overexpressing TAP-SUMO-2 or TAP-empty were used in conjunction with SILAC (Stable Isotope Labeling of Amino acids in Culture) (Golebiowski et al, 2009, 2010). Cells were grown in Dulbecco’s modified Eagle’s medium except that L-arginine and L-lysine were replaced with stable isotope (SILAC) forms depending on the treatment. Medium was supplemented with 10% dialyzed fetal calf serum. SILAC experiment compared TAP-containing cells (Lys0 and Arg0) with invasive Shigella flexneri (M90T) infected TAP-SUMO-2-containing cells (4,4,5,5-D4-lysine, Lys4, and 13C6- arginine, Arg6) and TAP-SUMO-2-containing cells infected with (mxiD) non-invasive strain of Shigella (13C6 15N2-lysine, Lys8, and 13C6 15N4 -arginine, Arg10).

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell

DISEASE(S): Shigellosis

SUBMITTER: Sabrina Fritah  

LAB HEAD: Anne Dejean

PROVIDER: PXD001100 | Pride | 2018-10-19

REPOSITORIES: Pride

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Dataset's files

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Action DRS
10_04_10_SF_TAP-SUMO_1.raw Raw
10_04_10_SF_TAP-SUMO_10.raw Raw
10_04_10_SF_TAP-SUMO_2.raw Raw
10_04_10_SF_TAP-SUMO_3.raw Raw
10_04_10_SF_TAP-SUMO_4.raw Raw
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Publications

Sumoylation controls host anti-bacterial response to the gut invasive pathogen Shigella flexneri.

Fritah Sabrina S   Lhocine Nouara N   Golebiowski Filip F   Mounier Joëlle J   Andrieux Alexandra A   Jouvion Grégory G   Hay Ronald T RT   Sansonetti Philippe P   Dejean Anne A  

EMBO reports 20140805 9

Shigella flexneri, the etiological agent of bacillary dysentery, invades the human colonic epithelium and causes its massive inflammatory destruction. Little is known about the post-translational modifications implicated in regulating the host defense pathway against Shigella. Here, we show that SUMO-2 impairs Shigella invasion of epithelial cells in vitro. Using mice haploinsufficient for the SUMO E2 enzyme, we found that sumoylation regulates intestinal permeability and is required to restrict  ...[more]