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Histione HCD and CID datasets for evaluation of search engines

ABSTRACT: To identify confident spectra from histone peptides containing PTMs, we present a method in which one kind of modification is searched each time. We then combine the identifications of multiple search engines to obtain confident results. We find that two search engines, pFind and Mascot, identify most of the confident results. This study will be beneficial those who are interested in histone proteomics analysis.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): T Cell, Cell Culture

SUBMITTER: Zuo-Fei Yuan

LAB HEAD: Benjamin A. Garcia

PROVIDER: PXD001118 | Pride | 2014-09-01

REPOSITORIES: pride

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			Action	DRS
	1HCD_Histone.mgf	Mgf
	1HCD_Histone.raw	Raw
	1HCD_Histone_1.dat	Other
	1HCD_Histone_1.dat-pride.pride.mgf.gz	Mgf
	1HCD_Histone_1.dat-pride.pride.mztab.gz	Mztab

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Publications

Evaluation of proteomic search engines for the analysis of histone modifications.

Yuan Zuo-Fei ZF Lin Shu S Molden Rosalynn C RC Garcia Benjamin A BA

Journal of proteome research 20140907 10

Identification of histone post-translational modifications (PTMs) is challenging for proteomics search engines. Including many histone PTMs in one search increases the number of candidate peptides dramatically, leading to low search speed and fewer identified spectra. To evaluate database search engines on identifying histone PTMs, we present a method in which one kind of modification is searched each time, for example, unmodified, individually modified, and multimodified, each search result is ...[more]

PMID: 25167464

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