Dataset Information

Oligosaccharide Substrate Preferences of Human Extracellular Sulfatase Sulf2

ABSTRACT: In this study, we characterized the substrate preferences of human HSulf2 using HS oligosaccharides with various lengths and sulfation degrees from several naturally occurring HS sources by applying liquid chromatography mass spectrometry based glycomics methods.

INSTRUMENT(S): 6520 Quadrupole Time-of-Flight LC/MS

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Small Intestine Mucosa

SUBMITTER: Yu Huang

LAB HEAD: Joseph Zaia

PROVIDER: PXD001157 | Pride | 2014-07-22

REPOSITORIES: Pride

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Oligosaccharide substrate preferences of human extracellular sulfatase Sulf2 using liquid chromatography-mass spectrometry based glycomics approaches.

Huang Yu Y Mao Yang Y Buczek-Thomas Jo Ann JA Nugent Matthew A MA Zaia Joseph J

PloS one 20140815 8

Sulfs are extracellular endosulfatases that selectively remove the 6-O-sulfate groups from cell surface heparan sulfate (HS) chain. By altering the sulfation at these particular sites, Sulfs function to remodel HS chains. As a result of the remodeling activity, HSulf2 regulates a multitude of cell-signaling events that depend on interactions between proteins and HS. Previous efforts to characterize the substrate specificity of human Sulfs (HSulfs) focused on the analysis of HS disaccharides and ...[more]

PMID: 25127119

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