Dataset Information

Quantitative Phosphorylation analysis in human platelets after ADP stimulation

ABSTRACT: Human platelets were treated with 20 µM of ADP for 10, 30 and 60 s in triplicate. In a second experiment blood donations of three donors were split again in four fractions and one was treated with 20 µM of ADP and the second and third with 20 µM of ADP for 30 s and subsequently with 2 nM of Iloprost for further 30 s and 60 s.

INSTRUMENT(S): LTQ Orbitrap Velos, Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Platelet, Platelet, Blood

SUBMITTER: Florian Beck

LAB HEAD: Florian Beck

PROVIDER: PXD001189 | Pride | 2017-03-14

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	ADPIloprostReplicate1TiO2HILIC30-35.raw	Raw
	ADPIloprostReplicate1TiO2HILIC36-37.raw	Raw
	ADPIloprostReplicate1TiO2HILIC38-39.raw	Raw
	ADPIloprostReplicate1TiO2HILIC40-41.raw	Raw
	ADPIloprostReplicate1TiO2HILIC42-43.raw	Raw

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Publications

Temporal quantitative phosphoproteomics of ADP stimulation reveals novel central nodes in platelet activation and inhibition.

Beck Florian F Geiger Jörg J Gambaryan Stepan S Solari Fiorella A FA Dell'Aica Margherita M Loroch Stefan S Mattheij Nadine J NJ Mindukshev Igor I Pötz Oliver O Jurk Kerstin K Burkhart Julia M JM Fufezan Christian C Heemskerk Johan W M JW Walter Ulrich U Zahedi René P RP Sickmann Albert A

Blood 20161109 2

Adenosine diphosphate (ADP) enhances platelet activation by virtually any other stimulant to complete aggregation. It binds specifically to the G-protein-coupled membrane receptors P2Y1 and P2Y12, stimulating intracellular signaling cascades, leading to integrin αIIbβ3 activation, a process antagonized by endothelial prostacyclin. P2Y12 inhibitors are among the most successful antiplatelet drugs, however, show remarkable variability in efficacy. We reasoned whether a more detailed molecular unde ...[more]

PMID: 28060719

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