Proteomics

Dataset Information

0

Multiple Proteases to Localize Oxidation Sites


ABSTRACT: Proteins present in cellular environments with high levels of reactive oxygen and nitrogen species and/or low levels of antioxidants are highly susceptible to oxidative post-translational modification (PTM). Irreversible oxidative PTMs can generate a complex distribution of modified protein molecules, recently termed as proteoforms. Using ubiquitin as a model system, we mapped oxidative modification sites using trypsin, Lys-C, and Glu-C peptides. Several M+16 Da proteoforms were detected as well as proteoforms that include other previously unidentified oxidative modifications. This work highlights the use of multiple protease digestions to give insights to the complexity of oxidative modifications possible in bottom-up analyses.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Bos Taurus (bovine)

SUBMITTER: Liqing Gu  

LAB HEAD: Rena A. S. Robinson

PROVIDER: PXD001204 | Pride | 2016-07-06

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
ESI_OX_UBI_Full.raw Raw
ESI_UM_UBI_Full.raw Raw
GluC.msf-pride.xml-merged.pride.mgf.gz Mgf
GluC.msf-pride.xml-merged.pride.mztab.gz Mztab
GluC.msf-pride.xml-merged.xml.gz Xml
Items per page:
1 - 5 of 15
altmetric image

Publications

Multiple proteases to localize oxidation sites.

Gu Liqing L   Robinson Renã A S RA  

PloS one 20150316 3


Proteins present in cellular environments with high levels of reactive oxygen and nitrogen species and/or low levels of antioxidants are highly susceptible to oxidative post-translational modification (PTM). Irreversible oxidative PTMs can generate a complex distribution of modified protein molecules, recently termed as proteoforms. Using ubiquitin as a model system, we mapped oxidative modification sites using trypsin, Lys-C, and Glu-C peptides. Several M+16 Da proteoforms were detected as well  ...[more]

Similar Datasets

2021-06-09 | PXD025496 | Pride
2020-01-27 | PXD000606 | Pride
2018-10-18 | PXD002966 | Pride
2022-02-24 | PXD003982 | Pride
2015-07-07 | PXD002197 | Pride
2020-03-05 | PXD014897 | Pride
2015-07-07 | PXD002201 | Pride
2018-06-18 | PXD009416 | Pride
2015-07-07 | PXD002199 | Pride
2015-07-07 | PXD002198 | Pride