Dataset Information

Tissue Engineered Rat Lung Proteomics

ABSTRACT: The goal of this project was to develop a method to fully characterize the proteome of native and decellularized rat lungs. In short, we show that chemical digestion is necessary yield the full complement of the insoluble proteins that make up decellularized scaffolds, a step that is generally ignored in the literature, but necessary to attain a representative readout of the tissue proteome

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Lung

SUBMITTER: Ryan Hill

LAB HEAD: Kirk Hansen

PROVIDER: PXD001213 | Pride | 2015-02-20

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20121108_Decel_Frac1_Lung01_Run01.raw	Raw
	20121108_Decel_Frac1_Lung01_Run01_ITMSms2cid.txt	Txt
	20121108_Decel_Frac1_Lung01_Run01_ITMSms2cid.txt (F063660_1).mzid_20121108_Decel_Frac1_Lung01_Run01_ITMSms2cid.txt_(F063660_1).pride.mgf.gz	Mzid
	20121108_Decel_Frac1_Lung01_Run01_ITMSms2cid.txt (F063660_1).pride.mztab.gz	Mztab
	20121108_Decel_Frac1_Lung01_Run01_ITMSms2cid.txtF063660_1.mzid.gz	Mzid

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Publications

Quantification of extracellular matrix proteins from a rat lung scaffold to provide a molecular readout for tissue engineering.

Hill Ryan C RC Calle Elizabeth A EA Dzieciatkowska Monika M Niklason Laura E LE Hansen Kirk C KC

Molecular & cellular proteomics : MCP 20150208 4

The use of extracellular matrix (ECM) scaffolds, derived from decellularized tissues for engineered organ generation, holds enormous potential in the field of regenerative medicine. To support organ engineering efforts, we developed a targeted proteomics method to extract and quantify extracellular matrix components from tissues. Our method provides more complete and accurate protein characterization than traditional approaches. This is accomplished through the analysis of both the chaotrope-sol ...[more]

PMID: 25660013

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