Proteomics

Dataset Information

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A High-resolution Systems-wide Screen for Substrates of the SCFβTrCP Ubiquitin Ligase - reproducibility evaluation

ABSTRACT: This project demonstrates a combined bioinformatics and affinity purification-mass spectrometry (AP-MS) workflow for identifying in a systems-wide manner bone fide substrates of βTrCP

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Kidney

SUBMITTER: Teck Yew Low  

LAB HEAD: Albert J.R. Heck

PROVIDER: PXD001224 | Pride | 2015-01-06

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
FBW11_Reproducibility_CRAPome_Results.xlsx Xlsx
OR2_090902_BTrCP_R447A_IP_Band01.RAW Raw
OR2_090902_BTrCP_R447A_IP_Band02.RAW Raw
OR2_090902_BTrCP_R447A_IP_Band03.RAW Raw
OR2_090902_BTrCP_R447A_IP_Band04.RAW Raw
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Publications

A systems-wide screen identifies substrates of the SCFβTrCP ubiquitin ligase.

Low Teck Yew TY   Peng Mao M   Magliozzi Roberto R   Mohammed Shabaz S   Guardavaccaro Daniele D   Heck Albert J R AJ  

Science signaling 20141216 356

Cellular proteins are degraded by the ubiquitin-proteasome system (UPS) in a precise and timely fashion. Such precision is conferred by the high substrate specificity of ubiquitin ligases. Identification of substrates of ubiquitin ligases is crucial not only to unravel the molecular mechanisms by which the UPS controls protein degradation but also for drug discovery purposes because many established UPS substrates are implicated in disease. We developed a combined bioinformatics and affinity pur  ...[more]