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Qualitative and quantitative analysis of chicken semen

ABSTRACT: Chicken proteome of ejaculated spermatozoa (SPZ) and seminal plasma (SP) and evaluation of the molecular phenotype related to sperm quality were investigated using GeLC-MS/MS approach.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Gallus Gallus (chicken)

SUBMITTER: Labas Valerie

LAB HEAD: Valérie Labas

PROVIDER: PXD001254 | Pride | 2014-08-22

REPOSITORIES: Pride

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			Action	DRS
	12-0309_Blesbois_spz_coq10_1_ORBI732.mzML	Mzml
	12-0309_Blesbois_spz_coq2_1_ORBI733.mzML	Mzml
	12-0309_Blesbois_spz_coq3_1_ORBI734.mzML	Mzml
	12-0309_Blesbois_spz_coq9_1_ORBI731.mzML	Mzml
	12-0409_Blesbois_spz_coq11_1_ORBI736.mzML	Mzml

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Publications

Qualitative and quantitative peptidomic and proteomic approaches to phenotyping chicken semen.

Labas Valérie V Grasseau Isabelle I Cahier Karine K Gargaros Audrey A Harichaux Grégoire G Teixeira-Gomes Ana-Paula AP Alves Sabine S Bourin Marie M Gérard Nadine N Blesbois Elisabeth E

Journal of proteomics 20140730

Understanding of the avian male gamete biology is essential to improve the conservation of genetic resources and performance in farming. In this study, the chicken semen peptidome/proteome and the molecular phenotype related to sperm quality were investigated. Spermatozoa (SPZ) and corresponding seminal plasma (SP) from 11 males with different fertilizing capacity were analyzed using three quantitative strategies (fluid and intact cells MALDI-MS, SDS-PAGE combined to LC-MS/MS with spectral count ...[more]

PMID: 25086240

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