Proteomics

Dataset Information

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Correction To - Confetti: A Multi-protease Map of the HeLa Proteome for Comprehensive Proteomics

ABSTRACT: Contains corrected HeLa SAX fractionated datasets for the Trypsin, LysC, and Chymotrypsin-Trypsin digests of the Confetti project. Original submission was PXD000900. Together with PXD000900 this submission makes up the dataset used for Confetti build 1.2.

INSTRUMENT(S): LTQ Orbitrap Elite, Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hela Cell

SUBMITTER: David Trudgian  

LAB HEAD: Hamid Mirzaei

PROVIDER: PXD001258 | Pride | 2014-08-28

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
Confetti_1_2_PSMs.txt Txt
Confetti_1_2_Proteins.txt Txt
Confetti_Correction_PSMs.txt Txt
Confetti_Correction_Proteins.txt Txt
HeLa_LysC_80pcACN_HCD_1_20140725.mzML Mzml
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Publications

Confetti: a multiprotease map of the HeLa proteome for comprehensive proteomics.

Guo Xiaofeng X   Trudgian David C DC   Lemoff Andrew A   Yadavalli Sivaramakrishna S   Mirzaei Hamid H  

Molecular & cellular proteomics : MCP 20140402 6

Bottom-up proteomics largely relies on tryptic peptides for protein identification and quantification. Tryptic digestion often provides limited coverage of protein sequence because of issues such as peptide length, ionization efficiency, and post-translational modification colocalization. Unfortunately, a region of interest in a protein, for example, because of proximity to an active site or the presence of important post-translational modifications, may not be covered by tryptic peptides. Detec  ...[more]