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Identification of miR-145 targets using SILAC

ABSTRACT: miR-145 is downregulated in multiple cancers. The introduction of miR-145 could alleviate the tumor burden in the pancreatic cancer mouse model. However, how miR-145 mediates the tumor suppression is still an open question. In this study, we aimed to identify the targets of miR-145 using a SILAC approach.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

DISEASE(S): Carcinoma

SUBMITTER: Akhilesh Pandey

LAB HEAD: Akhilesh Pandey

PROVIDER: PXD001260 | Pride | 2014-10-28

REPOSITORIES: Pride

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			Action	DRS
	Mir145_OG_1.raw	Raw
	Mir145_OG_10.raw	Raw
	Mir145_OG_11.raw	Raw
	Mir145_OG_12.raw	Raw
	Mir145_OG_13.raw	Raw

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Publications

Identification of miR-145 targets through an integrated omics analysis.

Huang Tai-Chung TC Renuse Santosh S Pinto Sneha S Kumar Praveen P Yang Yi Y Chaerkady Raghothama R Godsey Brian B Mendell Joshua T JT Halushka Marc K MK Civin Curt I CI Marchionni Luigi L Pandey Akhilesh A

Molecular bioSystems 20141030 1

MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression and protein synthesis. To characterize functions of miRNAs and to assess their potential applications, we carried out an integrated multi-omics analysis to study miR-145, a miRNA that has been shown to suppress tumor growth. We employed gene expression profiling, miRNA profiling and quantitative proteomic analysis of a pancreatic cancer cell line. In our transcriptomic analysis, overexpression of miR-145 was found to supp ...[more]

PMID: 25354783

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