Proteomics

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Investigating macrophage multinucleation by LC-MS/MS

ABSTRACT: Macrophage multinucleation (MM) is essential for various biological processes such as osteoclast-mediated bone resorption and multinucleated giant cell-associated inflammatory reactions. Here we study the molecular pathways underlying multinucleation in the rat through an integrative approach combining MS-based quantitative phosphoproteomics and transcriptome (high throughput RNA-sequencing) to identify new regulators of MM. We show that a strong metabolic shift towards HIF1-mediated glycolysis occurs at transcriptomic level during MM, together with modifications in phosphorylation of over 50 proteins including several ARF GTPase activators and polyphosphate inositol phosphatases. These results will provide a new framework for the combined analysis of transcriptional and post-translational changes during macrophage multinucleation, prioritizing essential genes and revealing the sequential events leading to the multinucleation of macrophages.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Bone Marrow, Macrophage

SUBMITTER: Peter Faull  

LAB HEAD: Peter Allen Faull

PROVIDER: PXD001269 | Pride | 2016-07-06

REPOSITORIES: Pride

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F001406.dat-pride.pride.mgf.gz Mgf
F001406.dat-pride.pride.mztab.gz Mztab
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F001407.dat-pride.pride.mgf.gz Mgf
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Publications

Integrating phosphoproteome and transcriptome reveals new determinants of macrophage multinucleation.

Rotival Maxime M   Ko Jeong-Hun JH   Srivastava Prashant K PK   Kerloc'h Audrey A   Montoya Alex A   Mauro Claudio C   Faull Peter P   Cutillas Pedro R PR   Petretto Enrico E   Behmoaras Jacques J  

Molecular & cellular proteomics : MCP 20141222 3

Macrophage multinucleation (MM) is essential for various biological processes such as osteoclast-mediated bone resorption and multinucleated giant cell-associated inflammatory reactions. Here we study the molecular pathways underlying multinucleation in the rat through an integrative approach combining MS-based quantitative phosphoproteomics (LC-MS/MS) and transcriptome (high-throughput RNA-sequencing) to identify new regulators of MM. We show that a strong metabolic shift toward HIF1-mediated g  ...[more]

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