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Off-target responses in the HeLa proteome subsequent to transient plasmid transfection using SILAC

ABSTRACT: We report a significant differential expression of proteins, all of which are associated with a viral infection response typified by Interferon type I/II. Notably the upregulated proteins may affect several vital cellular processes, including cell cycle control, translation and post-translational modification of proteins.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Cervix Carcinoma

SUBMITTER: Animesh Sharma

LAB HEAD: Geir Slupphaug

PROVIDER: PXD001278 | Pride | 2014-12-08

REPOSITORIES: Pride

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			Action	DRS
	131019_SILAC_LN_HEc_2.raw	Raw
	131019_SILAC_LN_HEc_3.raw	Raw
	131019_SILAC_LN_HEc_Transfected.raw	Raw
	131019_SILAC_LN_HN_2.raw	Raw
	131019_SILAC_LN_HN_3.raw	Raw

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Off-target responses in the HeLa proteome subsequent to transient plasmid-mediated transfection.

Hagen Lars L Sharma Animesh A Aas Per Arne PA Slupphaug Geir G

Biochimica et biophysica acta 20141105 1

Transient transfection of mammalian cells with plasmid expression vectors and chemical transfection reagents is widely used to study protein transport and dynamics as well as phenotypic alterations mediated by the overexpressed protein. Despite the undisputed impact of this technique, surprisingly little is known about the cellular effects mediated by the transfection process per se. Conceivably, off-target effects could have implications upon proteins or processes being studied and understandin ...[more]

PMID: 25448019

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