Proteomics

Dataset Information

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Huh-7 exosomes and HBV - Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh-7 Cells Using SILAC Labeling and LC-MS/MS


ABSTRACT: Hepatitis B virus (HBV) infection could cause hepatitis, liver cirrhosis and hepatocellular carcinoma. HBV-mediated pathogenesis is only partially understood, but X protein (HBx) reportedly possesses oncogenic potential. Exosomes are small membrane vesicles with diverse functions released by various cells including hepatocytes, and HBV harnesses cellular exosome biogenesis and export machineries for virion morphogenesis and secretion. Therefore, HBV infection might cause changes in exosome contents with functional implications for both virus and host. In this project, exosome protein content changes induced by HBV and HBx were quantitatively analyzed by SILAC/LC-MS/MS. Exosomes prepared from SILAC-labeled hepatoma cell line Huh-7 transfected with HBx, wildtype or HBx-null HBV replicon plasmids were analyzed by LC-MS/MS.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hepatocyte

SUBMITTER: Jing Liu  

LAB HEAD: Youhua Xie

PROVIDER: PXD001339 | Pride | 2014-10-09

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
AC4mqpar.xml Xml
BD4mqpar.xml Xml
HBV.pCH9-3091.fasta Fasta
HBx.ZX.fasta Fasta
Set1.zip Other
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Publications

Quantitative proteomic analysis of exosome protein content changes induced by hepatitis B virus in Huh-7 cells using SILAC labeling and LC-MS/MS.

Zhao Xue X   Wu Yanxin Y   Duan Jinlin J   Ma Yanchun Y   Shen Zhongliang Z   Wei Lili L   Cui Xiaoxian X   Zhang Junqi J   Xie Youhua Y   Liu Jing J  

Journal of proteome research 20141008 12


Hepatitis B virus (HBV) infection could cause hepatitis, liver cirrhosis, and hepatocellular carcinoma. HBV-mediated pathogenesis is only partially understood, but X protein (HBx) reportedly possesses oncogenic potential. Exosomes are small membrane vesicles with diverse functions released by various cells including hepatocytes, and HBV harnesses cellular exosome biogenesis and export machineries for virion morphogenesis and secretion. Therefore, HBV infection might cause changes in exosome cont  ...[more]

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