Dataset Information

Absolute protein quantification allows differentiation of cell specific metabolic routes and functions

ABSTRACT: Total protein approach (TPA) is a proteomic method which allows calculation of concentrations of individual proteins and groups of functionally related proteins in any protein mixture without spike-in standards. Using the two step digestion filter aided sample preparation method and LC-MS/MS analysis we generated comprehensive quantitative datasets of mouse intestinal mucosa, liver, red muscle fibers, brain, and of human plasma, erythrocytes, and tumor cells lines. We show that the TPA based quantitative data reflect well-defined and specific physiological functions of different organs and cells, for example nutrient absorption and transport in intestine, amino acid catabolism and bile secretion in liver, and contraction of muscle fibers. Focusing on key metabolic processes we compared metabolic capacities in different tissues and cells. In addition, we demonstrate quantitative differences in the mitochondrial proteomes. Providing insight into the abundances of mitochondrial metabolite transporters we demonstrate that their titers are well tuned to cell specific metabolic requirements. This study provides for the first time a comprehensive overview of the protein hardware mediating metabolism in different mammalian organs and cells. The presented approach can be applied to any other system to study biological processes.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)

TISSUE(S): Erythrocyte, Brain, Liver, Small Intestine Mucosa, Cell Line Cell

SUBMITTER: Mario Oroshi

LAB HEAD: Jacek R Wisniewski

PROVIDER: PXD001352 | Pride | 2014-12-15

REPOSITORIES: Pride

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			Action	DRS
	20120314_EXQ6_JRW_ERYT_1_L_01.raw	Raw
	20120314_EXQ6_JRW_ERYT_1_L_02.raw	Raw
	20120314_EXQ6_JRW_ERYT_1_L_03.raw	Raw
	20120314_EXQ6_JRW_ERYT_1_L_04.raw	Raw
	20120314_EXQ6_JRW_ERYT_1_T_05.raw	Raw

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Absolute protein quantification allows differentiation of cell-specific metabolic routes and functions.

Wiśniewski Jacek R JR Koepsell Hermann H Gizak Agnieszka A Rakus Dariusz D Rakus Dariusz D

Proteomics 20150217 7

Total protein approach (TPA) is a proteomic method that allows calculation of concentrations of individual proteins and groups of functionally related proteins in any protein mixture without spike-in standards. Using the two-step digestion-filter-aided sample preparation method and LC-MS/MS analysis, we generated comprehensive quantitative datasets of mouse intestinal mucosa, liver, red muscle fibers, brain, and of human plasma, erythrocytes, and tumor cells lines. We show that the TPA-based qua ...[more]

PMID: 25475432

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