Proteomics

Dataset Information

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Direct evidence of milk consumption from ancient human dental calculus, St Helena


ABSTRACT: This study investigated the consumption of milk products in the archaeological record, utilizing human dental calculus as a reservoir of dietary proteins from archaeological samples from across Eurasia. Protein extraction and generation of tryptic peptides from dental calculus was performed using a filter-aided sample preparation (FASP) protocol, modified for ancient samples, on 92 samples of archaeological dental calculus. Samples were extracted at three laboratories; the Functional Genomics Centre Zürich (FGCZ), the Centre for GeoGenetics at the National History Museum of Denmark, and BioArCh at the University of York. Sample extracts were sequenced (LC-MS/MS) using an LTQ-Orbitrap Velos (FGCZ), a Q-Exactive Hybrid Quadrupole Orbitrap and an LTQ-Orbitrap Elite (Central Proteomics Facility, Target Discovery Institute, Oxford).

INSTRUMENT(S): LTQ Orbitrap Elite, Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Dental Plaque

SUBMITTER: Jessica Hendy  

LAB HEAD: Matthew Collins

PROVIDER: PXD001357 | Pride | 2015-02-25

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
F116626_JH3_QE.dat Other
F116631_JH4_QE.dat Other
F116641_JH5_QE.dat Other
F116659_JH6_QE.dat Other
F116690_JH7_QE.dat Other
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Publications


Milk is a major food of global economic importance, and its consumption is regarded as a classic example of gene-culture evolution. Humans have exploited animal milk as a food resource for at least 8500 years, but the origins, spread, and scale of dairying remain poorly understood. Indirect lines of evidence, such as lipid isotopic ratios of pottery residues, faunal mortality profiles, and lactase persistence allele frequencies, provide a partial picture of this process; however, in order to und  ...[more]

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