Proteomics

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Aging Mouse Brain Mitochondria LC-MS/MS


ABSTRACT: This study investigates the proteomic alterations in brain mitochondria isolated from 5- (mature), 12- (old), and 24-month-old (aged) mice to examine normal "healthy" aging. Using quantitative mass-spectrometry based super-SILAC, our findings revealed global proteomic changes in brain mitochondria identifying several metabolic pathways including glycolysis, the tricarboxylic acid cycle, and oxidative phosphorylation. Overall, we found that the bioenergetic function of these mitochondria was preserved suggesting the proteomic alterations potentially allow for compensatory mechanisms to combat aging.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

SUBMITTER: Kelly Stauch  

LAB HEAD: Howard Fox

PROVIDER: PXD001370 | Pride | 2015-01-27

REPOSITORIES: Pride

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Publications

Proteomic analysis and functional characterization of mouse brain mitochondria during aging reveal alterations in energy metabolism.

Stauch Kelly L KL   Purnell Phillip R PR   Villeneuve Lance M LM   Fox Howard S HS  

Proteomics 20150210 9


Mitochondria are the main cellular source of reactive oxygen species and are recognized as key players in several age-associated disorders and neurodegeneration. Their dysfunction has also been linked to cellular aging. Additionally, mechanisms leading to the preservation of mitochondrial function promote longevity. In this study we investigated the proteomic and functional alterations in brain mitochondria isolated from mature (5 months old), old (12 months old), and aged (24 months old) mice a  ...[more]

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