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Phosphoproteome Analysis of Magnaporthe oryzae During Appressorium Development

ABSTRACT: The rice pathogen, Magnaporthe oryzae, undergoes a complex developmental process leading to formation of an appressorium prior to plant infection. In an effort to better understand phosphoregulation during appressorium development, a mass spectrometry-based study was undertaken. Phosphosites were identified from phosphoproteins from mycelia, conidia, germlings, and appressoria of the wild type and a ckpA mutant. The cyclic-AMP dependent protein kinase A (CPKA) is required for initial perception of the leaf surface, development of functional appressoria, and timely mobilization of storage reserves in conidia, including glycogen and lipid bodies.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Magnaporthe Oryzae (rice Blast Fungus) (pyricularia Oryzae)

TISSUE(S): Conidium, Mycelium

SUBMITTER: Ralph Dean

LAB HEAD: Dr. Ralph Dean

PROVIDER: PXD001389 | Pride | 2015-05-13

REPOSITORIES: Pride

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Publications

Phosphoproteome Analysis Links Protein Phosphorylation to Cellular Remodeling and Metabolic Adaptation during Magnaporthe oryzae Appressorium Development.

Franck William L WL Gokce Emine E Randall Shan M SM Oh Yeonyee Y Eyre Alex A Muddiman David C DC Dean Ralph A RA

Journal of proteome research 20150515 6

The rice pathogen, Magnaporthe oryzae, undergoes a complex developmental process leading to formation of an appressorium prior to plant infection. In an effort to better understand phosphoregulation during appressorium development, a mass spectrometry based phosphoproteomics study was undertaken. A total of 2924 class I phosphosites were identified from 1514 phosphoproteins from mycelia, conidia, germlings, and appressoria of the wild type and a protein kinase A (PKA) mutant. Phosphoregulation d ...[more]

PMID: 25926025

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