Proteomics

Dataset Information

0

Metaproteome, Deep Lake, Antarctica


ABSTRACT: Deep Lake is a hypersaline system in Antarctica (68°33’36.8S, 78°11’48.7E) that is so saline it remains liquid at –20°C (DeMaere et al 2013). The lake is dominated by haloarchaea, comprising a low-complexity community that differs greatly to warm-hot latitude hypersaline systems, is hierarchical structured, and supports a high level of intergenera gene exchange. Metaproteomics was performed on biomass that was collected in the austral summer of 2008 by sequential size fractionation (20 – 3 µm, 3 – 0.8 µm, 0.8 – 0.1 µm). The data were integrated to obtain a systems level view of the active host-virus interactions occurring in this novel aquatic Antarctic system. DeMaere MZ, Williams TJ, Allen MA, Brown MV, Gibson JA, Rich J, Lauro FM, Dyall-Smith M, Davenport KW, Woyke T, Kyrpides NC, Tringe SG, Cavicchioli R (2013) High level of intergenera gene exchange shapes the evolution of haloarchaea in an isolated Antarctic lake. Proc Natl Acad Sci USA 110: 16939-16944

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Environmental Samples

SUBMITTER: Bernhard Tschitschko  

LAB HEAD: Prof. Rick Cavicchioli

PROVIDER: PXD001436 | Pride | 2015-07-21

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
0m_0.1um_replicate_1.raw Raw
0m_0.1um_replicate_2.raw Raw
0m_0.8um_replicate_1.raw Raw
0m_0.8um_replicate_2.raw Raw
0m_3um_replicate_1.raw Raw
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Publications

Antarctic archaea-virus interactions: metaproteome-led analysis of invasion, evasion and adaptation.

Tschitschko Bernhard B   Williams Timothy J TJ   Allen Michelle A MA   Páez-Espino David D   Kyrpides Nikos N   Zhong Ling L   Raftery Mark J MJ   Cavicchioli Ricardo R  

The ISME journal 20150630 9


Despite knowledge that viruses are abundant in natural ecosystems, there is limited understanding of which viruses infect which hosts, and how both hosts and viruses respond to those interactions-interactions that ultimately shape community structure and dynamics. In Deep Lake, Antarctica, intergenera gene exchange occurs rampantly within the low complexity, haloarchaea-dominated community, strongly balanced by distinctions in niche adaptation which maintain sympatric speciation. By performing m  ...[more]

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