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Investigation of human TFIID subcomplexes 7TAF and 8TAF by cross-linking mass spectrometry

ABSTRACT: 7TAF and 8TAF complexes have been cross-linked with the bifunctional reagent bis(sulfosuccinimidyl)suberate, BS3 that targets mostly lysines . Cross-linked complexes were separated from non-cross-linked species by SDS-PAGE and the product bands were digested in-gel. The cross-linked peptides were analysed by mass spectrometry.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Juan Zou

LAB HEAD: Juri Rappsilber

PROVIDER: PXD001454 | Pride | 2015-07-20

REPOSITORIES: Pride

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			Action	DRS
	Zou_Rappsilber_7TAF_2014_01.raw	Raw
	Zou_Rappsilber_7TAF_2014_02.raw	Raw
	Zou_Rappsilber_7TAF_2014_peak.zip	Other
	Zou_Rappsilber_7TAF_2014_search.csv	Csv
	Zou_Rappsilber_8TAF_2014_01.raw	Raw

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Publications

Cytoplasmic TAF2-TAF8-TAF10 complex provides evidence for nuclear holo-TFIID assembly from preformed submodules.

Trowitzsch Simon S Viola Cristina C Scheer Elisabeth E Conic Sascha S Chavant Virginie V Fournier Marjorie M Papai Gabor G Ebong Ima-Obong IO Schaffitzel Christiane C Zou Juan J Haffke Matthias M Rappsilber Juri J Robinson Carol V CV Schultz Patrick P Tora Laszlo L Berger Imre I

Nature communications 20150114

General transcription factor TFIID is a cornerstone of RNA polymerase II transcription initiation in eukaryotic cells. How human TFIID-a megadalton-sized multiprotein complex composed of the TATA-binding protein (TBP) and 13 TBP-associated factors (TAFs)-assembles into a functional transcription factor is poorly understood. Here we describe a heterotrimeric TFIID subcomplex consisting of the TAF2, TAF8 and TAF10 proteins, which assembles in the cytoplasm. Using native mass spectrometry, we defin ...[more]

PMID: 25586196

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