Proteomics

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Use of SWATH mass spectrometry for quantitative proteomic investigation of Shewanella oneidensis MR-1 biofilms grown on graphite cloth electrodes


ABSTRACT: . In this study we show successful use of SWATH-MS for quantitative proteomic analysis of a microbial electrochemically active biofilm. Shewanella oneidensis MR-1 was grown on carbon cloth electrodes under continuous anodic electrochemical polarizations in a bioelectrochemical system. Using lactate as the electron donor, anodes serving as terminal microbial electron acceptors were operated at three different electrode potentials (+0.71V, +0.21V & -0.19V vs. SHE) and the development of catalytic activity was monitored by measuring the current traces over time. Once maximum current was reached (usually within 21-29 hours) the electrochemical systems were shut off and biofilm proteins were extracted from the electrodes for proteomic assessment.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Shewanella Oneidensis (strain Mr-1)

TISSUE(S): Biofilm

SUBMITTER: Christy Grobbler  

LAB HEAD: Dr. Philip L. Bond

PROVIDER: PXD001472 | Pride | 2016-08-10

REPOSITORIES: pride

Dataset's files

Source:
Action DRS
20131113_Christy_SWATH_-0.4_1.wiff Wiff
20131113_Christy_SWATH_-0.4_1.wiff.scan Wiff
20131113_Christy_SWATH_-0.4_2.wiff Wiff
20131113_Christy_SWATH_-0.4_2.wiff.scan Wiff
20131113_Christy_SWATH_-0.4_3.wiff Wiff
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Publications

Use of SWATH mass spectrometry for quantitative proteomic investigation of Shewanella oneidensis MR-1 biofilms grown on graphite cloth electrodes.

Grobbler Christy C   Virdis Bernardino B   Nouwens Amanda A   Harnisch Falk F   Rabaey Korneel K   Bond Philip L PL  

Systematic and applied microbiology 20141129 2


Quantitative proteomics from low biomass, biofilm samples is not well documented. In this study we show successful use of SWATH-MS for quantitative proteomic analysis of a microbial electrochemically active biofilm. Shewanella oneidensis MR-1 was grown on carbon cloth electrodes under continuous anodic electrochemical polarizations in a bioelectrochemical system (BES). Using lactate as the electron donor, anodes serving as terminal microbial electron acceptors were operated at three different el  ...[more]

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