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Proteomics Analysis of Soybean Hypocotyl during Recovery after Flooding Stress

ABSTRACT: Soybean (Glycine max L. cv Enrei) seeds were sterilized with 2% sodium hypochlorite solution and then thoroughly rinsed in water. The sterilized seeds were sown 4 cm inside surface of sand in 450 mL of quartz seedling cases wetted with 150 mL water and grown at 25°C and 70% humidity in a growth chamber (Sanyo, Tokyo, Japan) under white fluorescent light (160 μmol m-2 s-1, 16 h light period/day). Two-day-old soybeans were flooded for 2 days and hypocotyl samples were collected at the day of removal of flooding and then at 2 more points during the 4-day recovery period.

INSTRUMENT(S): LTQ XL

ORGANISM(S): Glycine Max

TISSUE(S): Plant Cell, Hypocotyl

DISEASE(S): Wounds And Injuries

SUBMITTER: Mudassar Khan

LAB HEAD: Setsuko Komatsu

PROVIDER: PXD001567 | Pride | 2015-05-26

REPOSITORIES: Pride

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			Action	DRS
	201.RAW	Raw
	201.mgf	Mgf
	201.msf	Msf
	201.pep.xml	Pepxml
	202.RAW	Raw

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Publications

Proteomic analysis of soybean hypocotyl during recovery after flooding stress.

Khan Mudassar Nawaz MN Sakata Katsumi K Komatsu Setsuko S

Journal of proteomics 20150327

Soybean is a nutritionally important crop, but exhibits reduced growth and yields under flooding stress. To investigate soybean responses during post-flooding recovery, a gel-free proteomic technique was used to examine the protein profile in the hypocotyl. Two-day-old soybeans were flooded for 2 days and hypocotyl was collected under flooding and during the post-flooding recovery period. A total of 498 and 70 proteins were significantly changed in control and post-flooding recovering soybeans, ...[more]

PMID: 25818724

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