Dataset Information

Widespread somatic L1 retrotransposition occurs early during gastrointestinal cancer evolution

ABSTRACT: Somatic L1 retrotransposition events have been shown to occur in epithelial cancers1-8. Here, we attempted to determine how early somatic L1 insertions occurred during the development of gastrointestinal (GI) cancers. Using L1-targeted resequencing (L1-seq), we studied different stages of four colorectal cancers arising from colonic polyps, seven pancreatic carcinomas, as well as seven gastric cancers. Surprisingly, we found somatic L1 insertions not only in all cancer types and metastases, but also in colonic adenomas, well-known cancer precursors. Some insertions were also present in low quantities in normal GI tissues, occasionally caught in the act of being clonally fixed in the adjacent tumors. Insertions in adenomas and cancers numbered in the hundreds and many were present in multiple tumor sections implying clonal distribution. Our results demonstrate that extensive somatic insertional mutagenesis occurs very early during the development of GI tumors, probably before dysplastic growth. We assessed the impact of somatic L1 insertions on the expression of the corresponding protein-coding genes by comparing protein abundance in the polyp with the highest number of somatic L1 insertions with that of its paired normal colon using mass spectrometry analysis. Of the 10 validated somatic insertions that were in protein coding regions in the polyp, two proteins – KIAA1217 and WARS2 – were downregulated in the adenoma >90% and >70%, respectively.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Colorectum, Colon

DISEASE(S): Colon Cancer

SUBMITTER: Szilvia Solyom

LAB HEAD: Haig H. Kazazian, Jr.

PROVIDER: PXD001626 | Pride | 2015-08-11

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	MSK_Szilvia_4TMT_bRP_01.raw	Raw
	MSK_Szilvia_4TMT_bRP_02.raw	Raw
	MSK_Szilvia_4TMT_bRP_03.raw	Raw
	MSK_Szilvia_4TMT_bRP_04.raw	Raw
	MSK_Szilvia_4TMT_bRP_05.raw	Raw

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Publications

Structural polypeptides of simian rotavirus SA11 and the effect of trypsin.

Espejo R T RT López S S Arias C C

Journal of virology 19810101 1

Analysis of purified simian rotavirus has shown that it contains fewer structural polypeptide classes than previously reported. Two polypeptides (molecular weights, 62,000 and 28,000) commonly found in purified rotaviruses were, in fact, produced by cleavage of a larger structural polypeptide (molecular weight, about 88,000) by trypsin, which is usually employed to increase the yield of rotaviruses in tissue culture. Trypsin-uncleaved, double-shelled rotaviruses are probably composed of only fiv ...[more]

PMID: 6260970

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