Proteomics

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Age-related cleavages of crystallins in human lens cortical fibre cells generate a plethora of endogenous peptides and high molecular weight complexes


ABSTRACT: Age-related breakdown of lenticular crystallins is associated with lenticular disorders such as cataract. Despite playing a critical role in maintaining lens homeostasis, the mechanism(s) and consequences of this phenomenon are not well understood. Utilising a proteomic-based approach, this study characterised 238 endogenous peptides derived from age-related crystallin breakdowns present in the cortical tissues of young, middle-age and old human lenses. Quantitative mass spectrometry analysis showed that the concentration of a prominent crystallin breakdown product in the lens increased significantly with age, which, coupled with the age-related increase in variety of the LMW crystallin peptide in the lens cortex, suggests that a major crystallin breakdown event taking place in the human lens cortex shortly after middle-age. In-depth analysis on the crystallin peptide terminal amino acids indicate the presence of trypsin-like proteolysis in the lens cortical cells, providing useful information on the mechanism(s) that contribute to crystallin breakdown in the aging human lens. Taken together, this work enhances our understanding on the age-related crystallin breakdown process in the cortical tissues of the human lens.

INSTRUMENT(S): QSTAR

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Lens Cortex

SUBMITTER: Xiaomin Song  

LAB HEAD: Xiaomin Song

PROVIDER: PXD001630 | Pride | 2016-09-29

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
FIG5.pdf Pdf
UOW_1 (F089248).mzid_UOW_1_(F089248).pride.mgf.gz Mzid
UOW_1 (F089248).pride.mztab.gz Mztab
UOW_1.mgf Mgf
UOW_1.pride.mgf.gz Mgf
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Publications

Age-related cleavages of crystallins in human lens cortical fiber cells generate a plethora of endogenous peptides and high molecular weight complexes.

Su Shih-Ping SP   Song Xiaomin X   Xavier Dylan D   Aquilina J Andrew JA  

Proteins 20150819 10


Low molecular weight peptides derived from the breakdown of crystallins have been reported in adult human lenses. The proliferation of these LMW peptides coincides with the earliest stages of cataract formation, suggesting that the protein cleavages involved may contribute to the aggregation and insolubilization of crystallins. This study reports the identification of 238 endogenous LMW crystallin peptides from the cortical extracts of four human lenses representing young, middle and old-age hum  ...[more]

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