Dataset Information

Aeropyrum pernix K1 LC-MSMS - Perchlorate and chlorate reduction by the Crenarchaeon Aeropyrum pernix and two thermophilic Firmicutes

ABSTRACT: The study investigated the ability of selected (hyper-)thermophilic prokaryotes to grow anaerobically by the reduction of perchlorate and chlorate. Physiological, genomic and proteome analyses suggest that the Crenarchaeon Aeropyrum pernix reduces (per)chlorate with a periplasmic enzyme related to nitrate reductases, while it lacks a functional chlorite-disproportionating enzyme (Cld). A. pernix seems to rely on the chemical reactivity of reduced sulfur compounds with the toxic intermediate chlorite to complete the pathway. The chemical oxidation of thiosulfate (in excessive amounts present in the medium) to sulfate and the concomitant release of chloride anions from the reduction of chlorite are the products of a biotic-abiotic (per)chlorate reduction pathway in A. pernix. The apparent absence of Cld in two other (per)chlorate-reducing microorganisms and their dependence on sulfide for (per)chlorate reduction is consistent with earlier-made observations on (per)chlorate-reducing Archaeoglobus fulgidus. All here discussed microorganisms use strategies for complete (per)chlorate reduction that differ from the physiology of classical (per)chlorate-reducing mesophiles.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Aeropyrum Pernix (strain Atcc 700893 / Dsm 11879 / Jcm 9820 / Nbrc 100138 / K1)

SUBMITTER: Martijn Pinkse

LAB HEAD: Martijn W. H. Pinkse

PROVIDER: PXD001683 | Pride | 2017-06-02

REPOSITORIES: Pride

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			Action	DRS
	20140218_ML_2139_01.raw	Raw
	20140218_ML_2139_01.raw (F001303).mzid_20140218_ML_2139_01.raw_(F001303).pride.mgf.gz	Mzid
	20140218_ML_2139_01.raw (F001303).pride.mztab.gz	Mztab
	20140218_ML_2139_01.rawF001303.mzid.gz	Mzid
	20140218_ML_2139_01.rawF001303.mzid_20140218_ML_2139_01.raw_F001303.MGF	Mzid

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Perchlorate and chlorate reduction by the Crenarchaeon Aeropyrum pernix and two thermophilic Firmicutes.

Liebensteiner Martin G MG Pinkse Martijn W H MW Nijsse Bart B Verhaert Peter D E M PD Tsesmetzis Nicolas N Stams Alfons J M AJ Lomans Bart P BP

Environmental microbiology reports 20151023 6

This study reports the ability of one hyperthermophilic and two thermophilic microorganisms to grow anaerobically by the reduction of chlorate and perchlorate. Physiological, genomic and proteome analyses suggest that the Crenarchaeon Aeropyrum pernix reduces perchlorate with a periplasmic enzyme related to nitrate reductases, but that it lacks a functional chlorite-disproportionating enzyme (Cld) to complete the pathway. Aeropyrum pernix, previously described as a strictly aerobic microorganism ...[more]

PMID: 26332065

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