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Temporary Organ Specific Quantitative Proteomic Analysis of Soybean under Flooding and Drought Stresses

ABSTRACT: To elucidate the mechanism of soybean responding to flooding and drought, organ specific proteomics and time course proteomics were carried out.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Glycine Max

TISSUE(S): Plant Cell, Hypocotyl, Cotyledon, Root, Root Tip

DISEASE(S): Wounds And Injuries

SUBMITTER: Wang Xin

LAB HEAD: Setsuko Komatsu

PROVIDER: PXD001687 | Pride | 2015-10-06

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	R120C.mgf	Mgf
	R120C.msf	Msf
	R120C.pep.xml	Pepxml
	R120C.raw	Raw
	R120H.mgf	Mgf

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Publications

Gel-free/label-free proteomic analysis of root tip of soybean over time under flooding and drought stresses.

Wang Xin X Oh MyeongWon M Sakata Katsumi K Komatsu Setsuko S

Journal of proteomics 20150912

Growth in the early stage of soybean is markedly inhibited under flooding and drought stresses. To explore the responsive mechanisms of soybean, temporal protein profiles of root tip under flooding and drought stresses were analyzed using gel-free/label-free proteomic technique. Root tip was analyzed because it was the most sensitive organ against flooding, and it was beneficial to root penetration under drought. UDP glucose: glycoprotein glucosyltransferase was decreased and increased in soybea ...[more]

PMID: 26376099

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Gel-Free/Label-Free Proteomic Analysis Uncovers the Roles of Endoplasmic Reticulum of Soybean Root Tip under Flooding and Drought Stresses

Project description:As a stresses sensitive crop, this study will give some clues to enhance soybean tolerance of flooding and drought stresses. To uncover the response mechanisms occurring in the ER triggered by flooding and drought, ER proteomics was performed using gel-free/label-free proteomic approach. Besides, glycoproteomics was also producted to investigate the ER funtion in glycoprotein synthesis under stresses

2017-04-03 | PXD003397 | Pride

Proteomic and morphological analyses of post-flooding recovery in soybean root exposed to aluminum oxide nanoparticles

Project description:To elucidate Al2O3 NPs role in recovery of soybean under flooding stress, gel-free proteomic technique was used. The proteomic analysis of root including hypocotyl at 2, and 4 days of flooding with 50 ppm Al2O3 NPs leading to subsequent removal as compared to flooding was performed.

2016-04-19 | PXD003372 | Pride

Quantitative proteomics reveals the flooding tolerant mechanism in soybean mutant and ABA treated soybean

Project description:Soybean, which provides abundant proteins and edible oil, is an important crop; however, it is very sensitive to flooding stress. The growth and grain yields of soybean are seriously reduced by flooding stress. To explore the initial flooding-tolerant mechanism of soybean, flooding-tolerant mutant line and ABA treated soybean were used. Because both material and treatment showed the flooding tolerance, the commonly changed proteins in plants under initial flooding stress were identified. To identify the common proteins, gel free/label free proteomic technique was used.

2019-01-09 | PXD003329 | Pride

Nuclear proteomics reavels the role of protein synthesis and chromatin in root tips of soybean under initial flooding stress

Project description:Flooding has a severe negative effect on cultivation in the early stage of soybean growth. To understand the mechanism in early-stage soybean responding to initial flooding stress, nuclear proteomic technique was used.

2017-04-03 | PXD002429 | Pride

Analysis of initial changes in the proteome of soybean root tip under flooding stress

2022-02-22 | PXD000689 | Pride

Quantitative Proteomics Reveals the Role of Protein Phosphorylation in Initial Changes of Soybean Root Tip under Flooding Stress

Project description:Flooding has a severe negative effect on cultivation in the early stage of soybean growth. To understand the role of protein phosphorylation in early-stage soybean responding to flooding stress, quantitative phosphoproteomic technique was used.

2014-11-13 | PXD000898 | Pride

Organ Specific Proteomics of Soybean under Flooding and Drought Stresses

Project description:Flooding and drought are adverse factors for soybean growth. To obtain better insight into the response mechanism of soybean under flooding and drought stresses, organ specificanalysis was performed using gel-free proteomic technique.

2017-04-26 | PXD005342 | Pride

Proteomic analysis of soybean root including hypocotyl during recovery after drought stress

Project description:Soybean is a nutritionally important crop that exhibits reductions in growth and yields under drought stress. To investigate soybean responses during post-drought recovery, a gel-free proteomic technique was used to examine the protein profile. Two-day-old soybeans were stressed with drought for 4 days, recovered for 4 days, and root including hypocotyl was collected under drought and during the recovery stage. Morphological analysis revealed that growth was suppressed under drought stress that recovered following stress removal. Malondialdehyde content was increased under drought stress but returned to normal during the recovery stage. A total of 792 and 888 proteins were identified in control and drought-stressed root including hypocotyl during recovery stage, respectively. Based on the proteomic and cluster analyses, peroxidase and aldehyde dehydrogenase were significantly changed at analyzed time points under drought stress and during recovery. The activity of peroxidase was decreased under drought stress but increased during recovery. The activity of aldehyde dehydrogenase was increased under drought stress but returned to normal during the recovery stage. These results suggest that peroxidase and aldehyde dehydrogenase play key role in post-drought recovery in soybean by scavenging toxic reactive oxygen species and reducing harmful aldehydes load.

2016-06-16 | PXD003650 | Pride

Application of Simulation Model to Uncover the Mechanisms of Flooding Tolerance and Difference between Flooding and Drought in Soybean

Project description:To decipher stress responses under flooding and drought as well as flooding-tolerant mechanisms in soybean, the mathematic biological approach of simulation model was applied. Furthermore, wild-type soybean with ABA treatment and flooding-tolerant mutant line, which displayed flooding tolerance, were used as the flooding-tolerant materials. Proteins acquired by gel-free/label-free proteomic technique were used for simulation model of responses under different stresses and flooding tolerant.

2018-10-26 | PXD005680 | Pride

Proteomic analysis of the effect of aluminum oxide nanoparticles on soybean root under flooding stress

Project description:Flooding stress has a negative impact on the soybean cultivation in early growth stages. In order to understand the effect of aluminum oxide nanoparticles on the soybean growth under flooding stress, quantitative proteomics technique was used.

2015-08-31 | PXD002005 | Pride

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