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Circadian rhythms study of the unicellular algae Ostreococcus tauri by shotgun proteomics

ABSTRACT: The overall set of experiments consist of sampling a culture of the unicellular algae Ostreococcus tauri (wild type) every 4 hours over a period of 24h (6 time points). Each time point was performed with 5 replicate culture flasks, the cultures were grown under a 12:12 hour cycle (light:dark).

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Ostreococcus Tauri

TISSUE(S): Cell Suspension Culture

SUBMITTER: Thierry Le Bihan

LAB HEAD: Thierry Le Bihan

PROVIDER: PXD001734 | Pride | 2022-12-01

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	130425_o1__02_ph130410_ot_LD08-1_B1.RAW	Raw
	130425_o1__03_ph130410_ot_LD20-1_B2.RAW	Raw
	130425_o1__04_ph130410_ot_LD04-1_B3.RAW	Raw
	130425_o1__05_ph130410_ot_LD00-1_B4.RAW	Raw
	130425_o1__06_ph130410_ot_LD16-1_B5.RAW	Raw

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Circadian rhythms study of the unicellular algae Ostreococcus tauri by shotgun proteomics

Project description:The overall set of experiments consist of sampling a culture of the unicellular algae Ostreococcus tauri (wild type) every 4 hours over a period of 24h (6 time points). Each time point was performed with 5 replicate culture flasks, the cultures were grown under a 12:12 hour cycle (light:dark).

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Project description:Genome wide rhythmic transcription under light/dark cycles is associated with sequential transcription of specific biological processes genes in Ostreococcus tauri. Transcriptional profiling of Ostreococcus tauri under light/dark cycles. In order to identify genes with a diurnal rhythm, cells entrained under 12:12 light/dark cycles were sampled every 3 hours for 27 hours with two overlapping time points at Time 9 (Light ON at Time 9; Light OFF at Time 21) in 3 independent experiments.

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