Dataset Information

Extinct Bison Latifrons Proteomics

ABSTRACT: Bone samples from several vertebrates were collected from the Ziegler Reservoir fossil site, in Snowmass Village, Colorado and processed for proteomics analysis. The specimens come from Pleistocene megafauna, Bison latifrons, dating back to 110 or 120 ka. Proteomics analysis using a simplified sample preparation procedure and tandem mass spectrometry (MS) was applied to obtain protein identifications. Several bioinformatics resources were used to obtain peptide identifications based on sequence homology to extant species with annotated genomes. With the exception of soil sample controls, all samples resulted in confident peptide identifications that mapped to collagen I. In addition, we analyzed a specimen from the extinct Bison latifrons that yielded peptide identifications mapping to over 33 bovine proteins. Our analysis resulted in extensive fibrillar collagen sequence coverage, including the identification of post-translational modifications. Hydroxylysine glucosylgalactosylation, a modification thought to be involved in collagen fiber formation and bone mineralization, was identified. Meta-analyses of data from other studies indicates that this modification may be enriched in well-preserved prehistoric samples. Additional peptide sequences from extracellular matrix (ECM) and non-ECM proteins have also been identified. These data provide a framework for analyzing ancient protein signatures in well-preserved fossil specimens, while also contributing novel insights into the molecular basis of organic matter preservation.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Cranium

SUBMITTER: Matthew Wither

LAB HEAD: Kirk Charles Hansen

PROVIDER: PXD001827 | Pride | 2015-07-17

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20110502_BoneTissue_HCD_01.raw	Raw
	20110502_BoneTissue_HCD_02.raw	Raw
	20120731_Bison_HCD_Run1_Frac01.raw	Raw
	20120731_Bison_HCD_Run1_Frac02.raw	Raw
	20120731_Bison_HCD_Run1_Frac03.raw	Raw

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Publications

Preserved Proteins from Extinct Bison latifrons Identified by Tandem Mass Spectrometry; Hydroxylysine Glycosides are a Common Feature of Ancient Collagen.

Hill Ryan C RC Wither Matthew J MJ Nemkov Travis T Barrett Alexander A D'Alessandro Angelo A Dzieciatkowska Monika M Hansen Kirk C KC

Molecular & cellular proteomics : MCP 20150506 7

Bone samples from several vertebrates were collected from the Ziegler Reservoir fossil site, in Snowmass Village, Colorado, and processed for proteomics analysis. The specimens come from Pleistocene megafauna Bison latifrons, dating back ∼ 120,000 years. Proteomics analysis using a simplified sample preparation procedure and tandem mass spectrometry (MS/MS) was applied to obtain protein identifications. Several bioinformatics resources were used to obtain peptide identifications based on sequenc ...[more]

PMID: 25948757

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