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Phosphorylation of Cohesin's Deposition Factor, Scc2 Regulates its Deposition Activity

ABSTRACT: Phosphorylated residues of the budding yeast Scc2 protein were identified using phosphopeptide enrichment and mass spectrometry.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

TISSUE(S): Cell Culture

SUBMITTER: Matthew Wither

LAB HEAD: Paul C. Megee

PROVIDER: PXD001830 | Pride | 2015-09-10

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	Asynchronous_cells_Scc2_band_excision.mzid.gz	Mzid
	Asynchronous_cells_Scc2_band_excision.pride.mztab.gz	Mztab
	Asynchronous_cells_Scc2_band_excision.raw	Raw
	Asynchronous_cells_Scc2_band_excision_ITMSms2cid.mgf	Mgf
	Asynchronous_cells_Scc2_band_excision_ITMSms2cid.pride.mgf.gz	Mgf

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Publications

Phosphorylation of the Scc2 cohesin deposition complex subunit regulates chromosome condensation through cohesin integrity.

Woodman Julie J Hoffman Matthew M Dzieciatkowska Monika M Hansen Kirk C KC Megee Paul C PC

Molecular biology of the cell 20150909 21

The cohesion of replicated sister chromatids promotes chromosome biorientation, gene regulation, DNA repair, and chromosome condensation. Cohesion is mediated by cohesin, which is deposited on chromosomes by a separate conserved loading complex composed of Scc2 and Scc4 in Saccharomyces cerevisiae. Although it is known to be required, the role of Scc2/Scc4 in cohesin deposition remains enigmatic. Scc2 is a phosphoprotein, although the functions of phosphorylation in deposition are unknown. We id ...[more]

PMID: 26354421

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Characterization of R-loop-interacting proteins in embryonic stem cells reveals roles in ribosomal RNA processing and gene expression

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Alpha-1-antitrypsin binds to the glucocorticoid receptor with anti-inflammatory and anti-mycobacterial significance in macrophages

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