Proteomics

Dataset Information

0

Phosphorylation of S.pombe Rap1 analysed by LC-MS/MS


ABSTRACT: Identification and semi-quantitation of phosphorylation sites on Schizosaccharomyces pombe DNA-binding protein Rap1. A Rap1 construct was cultured in homozygous diploid temperature-sensitive pat1-114 mutant strain of S. pombe cells (time points: 3.5 h and 4.5h), and was purified by anti-PK immunoprecipitation. The resulting samples were run into a 1D SDS-PAGE gel with the target band excised, tryptically digested and analysed by LC-MS/MS using a Velos Orbitrap mass spectrometer.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Schizosaccharomyces Pombe

TISSUE(S): Cell Culture, Diploid Cell

DISEASE(S): Disease Free

SUBMITTER: Andrew Thompson  

LAB HEAD: Andrew James Thompson

PROVIDER: PXD001841 | Pride | 2016-04-27

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
24577-01.msf Msf
24577.raw Raw
25157-01.msf Msf
25157.raw Raw
25161-01.msf Msf
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Publications

Telomere protein Rap1 is a charge resistant scaffolding protein in chromosomal bouquet formation.

Amelina Hanna H   Subramaniam Shaan S   Moiseeva Vera V   Armstrong Christine Anne CA   Pearson Siân Rosanna SR   Tomita Kazunori K  

BMC biology 20150610


<h4>Background</h4>Chromosomes reorganize in early meiotic prophase to form the so-called telomere bouquet. In fission yeast, telomeres localize to the nuclear periphery via interaction of the telomeric protein Rap1 with the membrane protein Bqt4. During meiotic prophase, the meiotic proteins Bqt1-2 bind Rap1 and tether to the spindle pole body to form the bouquet. Although it is known that this polarized chromosomal arrangement plays a crucial role in meiotic progression, the molecular mechanis  ...[more]

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