Dataset Information

Light-sensing via H2O2 and a peroxiredoxin

ABSTRACT: Light controls control a vast array of biological processes, including cell and organelle motility, stress responses, organismal development and the entrainment of circadian rhythms, that maintain diurnal cycles of activity in organisms from cyanobacteria to humans. Recent studies indicate that a type of antioxidant and signaling proteins, peroxiredoxins, sustain circadian rhythms independent of characterized circadian pacemakers in organisms from all the three kingdoms of life, suggesting a role for H2O2 production in circadian clocks. Whereas many circadian clocks involve photosensitive pigments such as melanopsin and cryptochromes it is unclear whether peroxiredoxins can respond to light stimuli and how they interact with global signaling networks regulating e.g. clocks and aging, such as cyclic AMP (cAMP)/protein kinase A (PKA). In yeast, that lacks decidated photoreceptors, blue light induces cAMP-PKA-dependent, nuclear accumulation of a transcription factor, Msn2. However, the mechanism by which light represses pathway activity to stimulate Msn2 nuclear translocation is unknown. Here we identify increased H2O2–production via a conserved peroxisomal oxidase as the cause of light-induced Msn2 nuclear concentration. The H2O2 signal is transduced by the catalytic cysteines of the peroxiredoxin Tsa1 that relieve Msn2 from inhibitory PKA phosphorylation causing its nuclear accumulation. We propose that yeast senses light via H2O2 and a peroxiredoxin to inhibit cAMP/PKA activity and our data form a framework for the study of light responses in cells lacking dedicated light receptors and cAMP-controlled biological rhythms in multicellular organisms.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Wolfgang Reiter

LAB HEAD: Wolfgang Ludwig Reiter

PROVIDER: PXD001853 | Pride | 2018-07-03

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Rawfile_MS3_MSA_26022016.xlsx	Xlsx
	rep1_SILAC_Msn2_02mM_H2O2_10min_try_10_Reiter_20120418.raw	Raw
	rep1_SILAC_Msn2_02mM_H2O2_Reiter_fwd.msf	Msf
	rep1_SILAC_Msn2_02mM_H2O2_Reiter_fwd.pep.xml	Pepxml
	rep1_SILAC_Msn2_02mM_H2O2_tio2_Reiter_20120508.raw	Raw

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Publications

Light-sensing via hydrogen peroxide and a peroxiredoxin.

Bodvard Kristofer K Peeters Ken K Roger Friederike F Romanov Natalie N Igbaria Aeid A Welkenhuysen Niek N Palais Gaël G Reiter Wolfgang W Toledano Michel B MB Käll Mikael M Molin Mikael M

Nature communications 20170324

Yeast lacks dedicated photoreceptors; however, blue light still causes pronounced oscillations of the transcription factor Msn2 into and out of the nucleus. Here we show that this poorly understood phenomenon is initiated by a peroxisomal oxidase, which converts light into a hydrogen peroxide (H2O2) signal that is sensed by the peroxiredoxin Tsa1 and transduced to thioredoxin, to counteract PKA-dependent Msn2 phosphorylation. Upon H2O2, the nuclear ret ...[more]

PMID: 28337980

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