Dataset Information

ITRAQ-based Subcellular Proteome of Avibirnavirus-infected DF-1 Cells

ABSTRACT: Infectious bursal disease virus (IBDV) enters the host cells via endocytic pathway to achieve viral replication in the cytoplasm. We performed liquid chromatography-tandem mass spectrometry coupled with isobaric tags for relative and absolute quantification (iTRAQ) labeling of differential abundant protein species of IBDV-infected cells using a subcellular fractionation strategy. We find that viral infection regulates the expression and/or subcellular localization of more than one thousand host proteins in either the nuclear or the cytoplasmic fraction at the early phase of infection. These data provide clues to further understanding the replication and pathogenesis of IBDV and virus-host interactions.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Gallus Gallus (chicken)

TISSUE(S): Cell Culture, Fibroblast

SUBMITTER: Yanting Sun

LAB HEAD: Jiyong Zhou

PROVIDER: PXD001869 | Pride | 2020-05-26

REPOSITORIES: Pride

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iTRAQ-based quantitative subcellular proteomic analysis of Avibirnavirus-infected cells.

Sun Yanting Y Hu Boli B Fan Chengfei C Jia Lu L Zhang Yina Y Du Aifang A Zheng Xiaojuan X Zhou Jiyong J

Electrophoresis 20150617 14

Infectious bursal disease virus (IBDV) enters the host cells via endocytic pathway to achieve viral replication in the cytoplasm. Here, we performed LC-MS/MS coupled with isobaric tags for relative and absolute quantification labeling of differentially abundant proteins of IBDV-infected cells using a subcellular fractionation strategy. We show that the viral infection regulates the abundance and/or subcellular localization of 3211 proteins during early infection. In total, 23 cellular proteins i ...[more]

PMID: 25929241

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Project description:RNA-Seq was used to unveil the transcriptional profile of DF-1 cells at the early stage of cell-adapted Infectious Bursal Disease Virus (caIBDV) infection. Total RNAs were extracted from virus-infected cells at 0, 6 and 12 hpi. RNA-Seq datasets of respective samples mapped to 56.5 - 57.6% of isoforms in the reference genome Galgal4.73. At 6 hpi, 23 isoforms underwent an elevated expression, while 128 isoforms were up-regulated and 5 were down-regulated at 12 hpi in the virus-infected group. Besides, 10 isoforms were exclusively expressed in the virus-infected cells. Though no significant change was detected in cytokine and interferon expression levels at the first 12 hours of infection, modulations of the upstream regulators were observed. In addition to the reported regulatory factors including EIF2AK2, MX, OAS*A, GBP7 and IFIT, IBDV infection also triggered a IFIT5-IRF1/3-RSAD5 pathway in the DF-1 cells which potentially restricted the viral replication cycle in the early infection stage. Over-expression of LIPA and CH25H, together with the suppression of STARD4, LSS and AACS genes implied a modulation of membrane fluidity and lipid raft arrangement in the infected cells. Alternative splicing of the EFR3 homolog A gene was also through to be involved in the lipid membrane regulation, and these cumulative responses projected an inhibition of viral endocytosis. Recognition of viral RNA genomes and intermediates was presumably enhanced by the elevated levels of IFIH1, DHX58 and TRIM25 genes which possess properties on detecting viral dsRNA. On the other hand, the caIBDV arrested the host's apoptotic process by inducing the expression of apoptosis inhibitors including NFKBIA/Z, TNFAIP2/3 and ITA at the first 12 hours of infection. In conclusion, the differential expression landscape demonstrated with RNA-Seq provides a comprehensive picture on the molecular interactions between host cells and virus at the early stage of infection. Two sample groups (infected and mock-infected) at three time points (0, 6, 12 hpi) were analysed.

			Action	DRS
	F1_SCX-1.mgf	Mgf
	F1_SCX-1.raw	Raw
	F1_SCX-2.mgf	Mgf
	F1_SCX-2.raw	Raw
	F1_SCX-3.mgf	Mgf