Proteomics

Dataset Information

0

Analysis of Plasma Membrane, Endosomal and Cytoplasmic fractions of mouse embryonic fibroblast

ABSTRACT: This project aimed to identify novel endosomal proteins and therefore cellular fractionation experiments were performed. Adherent mouse embryonic fibroblast were scraped on ice into hypotonic lysis buffer (10 mM HEPES-KOH pH 7.2, 0.25 M sucrose, 1 mM EDTA, 1 mM MgOAc and protease & phosphatase inhibitors (PhosSTOP and Complete mini tablets from Roche). Cell membranes were fragmented with French Press and nuclei removed with 10 min 1000 x g centrifugation. Plasma membrane fraction was collected with 10 min 10 000 x g centrifugation and endosomal/cytoplasmic fractions with 1h 100,000 x g centrifugation. Membrane fractions were washed at least once with lysis buffer and cytoplasmic fractions were centrifuged twice. All fractionation steps were performed at +4°C or on ice. All fractions were dissolved in sample buffer for immunoblotting

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Embryo, Fibroblast

SUBMITTER: Guillaume Jacquemet  

LAB HEAD: Johanna Ivaska

PROVIDER: PXD001870 | Pride | 2015-10-14

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
141111_Guillaume_101-110 plasma membrane R1_ mouse.dat-pride.pride.mgf.gz Mgf
141111_Guillaume_101-110 plasma membrane R1_ mouse.dat-pride.pride.mztab.gz Mztab
141111_Guillaume_101-110plasmamembraneR1_mouse.dat-pride.xml.gz Xml
141111_Guillaume_201-210 cytosol R1 mouse.dat-pride.pride.mgf.gz Mgf
141111_Guillaume_201-210 cytosol R1 mouse.dat-pride.pride.mztab.gz Mztab
Items per page:
1 - 5 of 24
altmetric image

Publications

Integrin endosomal signalling suppresses anoikis.

Alanko Jonna J   Mai Anja A   Jacquemet Guillaume G   Schauer Kristine K   Kaukonen Riina R   Saari Markku M   Goud Bruno B   Ivaska Johanna J  

Nature cell biology 20151005 11

Integrin-containing focal adhesions transmit extracellular signals across the plasma membrane to modulate cell adhesion, signalling and survival. Although integrins are known to undergo continuous endo/exocytic traffic, the potential impact of endocytic traffic on integrin-induced signals is unknown. Here, we demonstrate that integrin signalling is not restricted to cell-ECM adhesions and identify an endosomal signalling platform that supports integrin signalling away from the plasma membrane. W  ...[more]

Similar Datasets

Other m6A profiles in shCTRL, shMETTL3 HeLa cells
2019-10-15 | GSE134103 | GEO
Methylation profiling RNA-BisSeq for YBX1 RIP in normal T24 cell
2019-07-02 | GSE133625 | GEO
Other ALKBH3 CLIP-seq in HeLa cells.
2022-03-01 | GSE112625 | GEO
Proteomics Ubiquitination in treated T. cruzi parasites
2022-02-17 | PXD027524 | Pride
Genomics Genome wide mapping of DDX41 chromatin binding using a targeted nuclease approach in U2OS cells after expression
2021-11-17 | GSE183083 | GEO
Genomics Snip1 and PRC2 coordinate the intrinsic apoptosis, cell division, and neurogenesis in the developing brain [CUT&RUN]
2023-07-12 | GSE212444 | GEO
Proteomics Interactome analysis of human Rab11a with proteins from influenza A virus infected cell lysates
2022-01-17 | PXD030058 | Pride
Other Human Argonaute Immunoaffinity Purification-associated RNA
2009-11-10 | GSE18834 | GEO
Proteomics SERRATE interacts with the nuclear exosome targeting complex to degrade primary miRNA precursors in Arabidopsis
2020-04-15 | PXD014447 | Pride
Proteomics Human Cell 293T BCAT1 IP GC-LC-MS
2024-11-15 | PXD041556 | Pride