Proteomics

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The prion protein contributes to epithelial-to-mesenchymal transition in the mouse NMuMG epithelial cell model

ABSTRACT: The physiological function of the prion protein (PrP) has remained elusive despite its widely recognized role in neurodegenerative diseases and sustained efforts to understand its molecular biology. On the basis of its evolutionary relationship to ZIP zinc transporters, protein-protein interactions it engages in, and the characteristics of a gastrulation arrest phenotype in a PrP-deficient model, we considered that PrP may contribute to the morphogenetic reprogramming of cells underlying epithelial-to-mesenchymal (EMT) transitions. We have conducted three global proteomics analyses to (1) identify proteins whose levels are changed during EMT in wild-type NMuMG cells; and determine proteins whose levels are affected by (2) stable knockdown or (3) transient knockdown of the prion protein relative to levels observed in wild-type NMuMG cells. We observed a highly significant correlation between proteins, which are changed during EMT or following PrP knockdown.

INSTRUMENT(S): Orbitrap Fusion ETD

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Disease Free

SUBMITTER: Gerold Schmitt-Ulms  

LAB HEAD: Gerold Schmitt-Ulms

PROVIDER: PXD001875 | Pride | 2015-07-10

REPOSITORIES: Pride

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18_OF_NMuMG_transient_wt_0_global_48h_TGFbeta_6_7_8_9_0_1_C18_1-3_SCX_1-3_Percolator.msf Msf
18_OF_NMuMG_wt_0h_48h_TGFbeta_global_6_7_8_9_0_1_C18_1.raw Raw
18_OF_NMuMG_wt_0h_48h_TGFbeta_global_6_7_8_9_0_1_C18_2.raw Raw
18_OF_NMuMG_wt_0h_48h_TGFbeta_global_6_7_8_9_0_1_C18_3.raw Raw
18_OF_NMuMG_wt_0h_48h_TGFbeta_global_6_7_8_9_0_1_SCX_1.raw Raw
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Publications

The Prion Protein Controls Polysialylation of Neural Cell Adhesion Molecule 1 during Cellular Morphogenesis.

Mehrabian Mohadeseh M   Brethour Dylan D   Wang Hansen H   Xi Zhengrui Z   Rogaeva Ekaterina E   Schmitt-Ulms Gerold G  

PloS one 20150819 8

Despite its multi-faceted role in neurodegenerative diseases, the physiological function of the prion protein (PrP) has remained elusive. On the basis of its evolutionary relationship to ZIP metal ion transporters, we considered that PrP may contribute to the morphogenetic reprogramming of cells underlying epithelial-to-mesenchymal transitions (EMT). Consistent with this hypothesis, PrP transcription increased more than tenfold during EMT, and stable PrP-deficient cells failed to complete EMT in  ...[more]