Proteomics

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Proteomic Study of Different Culture Medium Serum Volume Fractions on RANKL-dependent Differentiating into Osteoclasts of RAW264.7 Cells

ABSTRACT: Supplemental data for the article: Proteomic Study of Different Culture Medium Serum Volume Fractions on RANKL-dependent Differentiating into Osteoclasts of RAW264.7 Cells

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture

SUBMITTER: Wei Ge  

LAB HEAD: Wei Ge

PROVIDER: PXD001935 | Pride | 2015-06-02

REPOSITORIES: Pride

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MOUSE20140709.fasta Fasta
MS140676-1.raw Raw
MS140676-10.raw Raw
MS140676-11.raw Raw
MS140676-12.raw Raw
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Publications

Proteomic study of different culture medium serum volume fractions on RANKL-dependent RAW264.7 cells differentiating into osteoclasts.

Xiong Qi Q   Zhang Lihai L   Xin Lingli L   Gao Yanpan Y   Peng Ye Y   Tang Peifu P   Ge Wei W  

Proteome science 20150502

<h4>Background</h4>Cultivation of osteoclasts is a basic tool for investigating osteolytic bone diseases. Fetal bovine serum (FBS) is the standard supplement used for in vitro cell culture medium. Typically, the serum volume fraction used for osteoclast cultivation is 10%. In this study, we investigated the use of a low serum (1% FBS) model for culturing osteoclasts.<h4>Results</h4>To confirm the validity of this model for use in osteoclast research, we compared the capacity for osteoclastogenes  ...[more]