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Quantitative Proteomic Analysis of Catharanthus roseus with Induction of Indole Alkaloids

ABSTRACT: Catharanthus roseus produces a variety of indole alkaloids with significant biological activities. The indole alkaloids including catharanthine, vindolinine, ajmalicine and the precursor strictosidine were dramatically induced in the leaves following binary stress. To profile the modification of indole alkaloids in C. roseus seedlings under the binary stress of ultraviolet-B irradiation and dark incubation, gel-free proteomic analysis was carried out to uncover the underlying molecular mechanism.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Catharanthus Roseus Var. Roseus

TISSUE(S): Plant Cell, Leaf

SUBMITTER: xiaojian Yin

LAB HEAD: Setsuko Komatsu

PROVIDER: PXD001937 | Pride | 2015-08-24

REPOSITORIES: Pride

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			Action	DRS
	C2.RAW	Raw
	C2.mgf	Mgf
	C2.msf	Msf
	C2.pep.xml	Pepxml
	C3.RAW	Raw

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Publications

Binary stress induces an increase in indole alkaloid biosynthesis in Catharanthus roseus.

Zhu Wei W Yang Bingxian B Komatsu Setsuko S Lu Xiaoping X Li Ximin X Tian Jingkui J

Frontiers in plant science 20150728

Catharanthus roseus is an important medicinal plant, which produces a variety of indole alkaloids of significant pharmaceutical relevance. In the present study, we aimed to investigate the potential stress-induced increase of indole alkaloid biosynthesis in C. roseus using proteomic technique. The contents of the detectable alkaloids ajmalicine, vindoline, catharanthine, and strictosidine in C. roseus were significantly increased under binary stress. Proteomic analysis revealed that the abundanc ...[more]

PMID: 26284098

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A modular system regulates specialized metabolite pathway branch choice in the medicinal plant Catharanthus roseus

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A modular system regulates specialized metabolite pathway branch choice in the medicinal plant Catharanthus roseus

Project description:This dataset belongs to a set of three RNA-Seq experiments that were carried out to study the regulation of monoterpenoid indole alkaloid production in the medicinal plant Catharanthus roseus. For this dataset, C. roseus stems were dissected to separate the epidermis from the lower tissues. Leaves were dissected to obtain veins and veinless leaves. As controls, undissected leaves and stems were used. Three biological replicates were analyzed per sample.

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Project description:Leaf is sensitive to environmental changes and exhibits specific responses to abiotic stress. To identify the response mechanism in soybean leaf under waterlogging stress, a gel-free/label-free proteomic technique combined with polyethylene glycol fractionation was used.

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Proteomics Analysis of Soybean Hypocotyl during Recovery after Flooding Stress

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