Dataset Information

Phosphoproteomics of Human ACHN cells by estrogen

ABSTRACT: To find out the potential targets in response to estrogen treatment on ACHN cell, a human renal cell carcinoma (RCC) cell line. Since estrogen can repress ACHN growth in a partly estrogen receptor-dependent manner, it is possible that phosphorylation state in ACHN cells is regulated.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Renal Cell Carcinoma

SUBMITTER: Wei-Chi Ku

LAB HEAD: Wei-Chi Ku

PROVIDER: PXD001962 | Pride | 2016-04-26

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	120807ku-05-Est1-Pho-1.raw	Raw
	120807ku-07-Est1-Pho-2.raw	Raw
	120807ku-09-Est1-Am-1.raw	Raw
	120807ku-11-Est1-Am-2.raw	Raw
	120807ku-13-Est1-Py-1.raw	Raw

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Publications

Dual Roles of 17-β Estradiol in Estrogen Receptor-dependent Growth Inhibition in Renal Cell Carcinoma.

Chen Kuo-Chiang KC Lin Chih-Ming CM Huang Chi-Jung CJ Chen Shao-Kuan SK Wu Sheng-Tang ST Chiang Han-Sun HS Ku Wei-Chi WC

Cancer genomics & proteomics 20160501 3

<h4>Background</h4>It has been proposed that 17-β-estradiol (E2) activates estrogen receptor and inhibits renal cell carcinoma (RCC) growth. In the present study we explored the role of E2 and ER in the regulation of RCC growth.<h4>Materials and methods</h4>The RCC cell line ACHN was treated by E2 combining with E2 antagonist Fulvestrant or ER knockdown, and cell growth was monitored. Quantitative phosphoproteomics was applied to study the E2 regulated non-genomic phosphorylation changes. Wester ...[more]

PMID: 27107064

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