Ontology highlight
ABSTRACT:
OTHER RELATED OMICS DATASETS IN: GSE67110MSV000079767PRJNA279194PRJNA278966PRJNA278972PRJNA278971
INSTRUMENT(S): LTQ Orbitrap Elite
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Colon Epithelial Cell, Cell Culture, Colon
DISEASE(S): Colon Cancer
SUBMITTER: Friedel Drepper
LAB HEAD: Bettina Warscheid
PROVIDER: PXD001976 | Pride | 2015-07-23
REPOSITORIES: Pride
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Molecular & cellular proteomics : MCP 20150716 10
We determined the effect of p53 activation on de novo protein synthesis using quantitative proteomics (pulsed stable isotope labeling with amino acids in cell culture/pSILAC) in the colorectal cancer cell line SW480. This was combined with mRNA and noncoding RNA expression analyses by next generation sequencing (RNA-, miR-Seq). Furthermore, genome-wide DNA binding of p53 was analyzed by chromatin-immunoprecipitation (ChIP-Seq). Thereby, we identified differentially regulated proteins (542 up, 56 ...[more]