Proteomics

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Secretome of P2X7 receptor - Macrophage activation and polarization modify P2X7 receptor secretome influencing the inflammatory process

ABSTRACT: In order to determine P2X7R secretome we analyzed the proteins present in cell-free supernatants from wild-type (P2rx7+/+) or P2rx7-/- bone marrow-derived macrophages (BMDMs) polarized either to M1 or M2 and subsequently treated with ATP. BMDMs were primed with LPS (M1) or IL-4 (M2) for 4 hours and the proteins secreted during this step were extensively washed with PBS before ATP was added in fresh buffer. The complex mixture of proteins obtained in the macrophages supernatants after ATP stimulation were fractionated using one dimension gel electrophoresis and 10 bands were selected for LC-MS/MS analysis based in their presence in higher intensity in P2rx7+/+ supernatant compared with P2rx7-/- supernatant.

INSTRUMENT(S): Thermo Finnigan instrument model

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Bone Marrow, Macrophage

SUBMITTER: Carlos de Torre Minguela  

LAB HEAD: Carlos de Torre Minguela

PROVIDER: PXD001981 | Pride | 2016-03-11

REPOSITORIES: Pride

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Dataset's files

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Action DRS
10KOIL4_SDSPAGE_071212.RAW Raw
10KOIL4_SDSPAGE_071212.msf Msf
10KO_SDSPAGE_062112.RAW Raw
10KO_SDSPAGE_062112.msf Msf
10KO_SDSPAGE_070312.RAW Raw
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Publications

Macrophage activation and polarization modify P2X7 receptor secretome influencing the inflammatory process.

de Torre-Minguela Carlos C   Barberà-Cremades Maria M   Gómez Ana I AI   Martín-Sánchez Fátima F   Pelegrín Pablo P  

Scientific reports 20160303

The activation of P2X7 receptor (P2X7R) on M1 polarized macrophages induces the assembly of the NLRP3 inflammasome leading to the release of pro-inflammatory cytokines and the establishment of the inflammatory response. However, P2X7R signaling to the NLRP3 inflammasome is uncoupled on M2 macrophages without changes on receptor activation. In this study, we analyzed P2X7R secretome in wild-type and P2X7R-deficient macrophages polarized either to M1 or M2 and proved that proteins released after P  ...[more]

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