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Development of a Mass Spectrometry-Based Quantitation Assay for Human Body Fluid Proteins using a Wheat Germ Cell-Free Synthesis System

ABSTRACT: In this study, we have developed a large-scale SRM assay targeting human body fluid proteins based on publicly available proteomics data. We first aimed to produce, in a high throughput manner, a peptide reference library. For this purpose, we selected 2,201 targeted peptides representing 1,215 proteins from previous published studies, and in vitro synthesized them as peptide concatamers (QconCATs) using a wheat germ cell-free system.

INSTRUMENT(S): Q TRAP

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Nobuaki Takemori

LAB HEAD: Nobuaki Takemori

PROVIDER: PXD002090 | Pride | 2016-05-24

REPOSITORIES: Pride

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			Action	DRS
	HBF_001_IPI_Human.group	Other
	HBF_001_IPI_Human.group.xml	Xml
	HBF_001_IPI_Human__FDR.xlsx	Xlsx
	HBF_002_IPI_Human.group	Other
	HBF_002_IPI_Human.group.xml	Xml

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Publications

High-throughput production of a stable isotope-labeled peptide library for targeted proteomics using a wheat germ cell-free synthesis system.

Takemori Nobuaki N Takemori Ayako A Tanaka Yuki Y Ishizaki Jun J Hasegawa Hitoshi H Shiraishi Atsushi A Ohashi Yuichi Y

Molecular bioSystems 20160701 8

Quantitative proteomic approaches using selected reaction monitoring (SRM) are currently limited by the difficulty in the preparation of reference standards. In this study, we demonstrat the high-throughput production of a reference peptide library using a wheat germ cell-free synthesis system to develop a large-scale SRM assay for targeted proteomics. ...[more]

PMID: 27203355

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