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California sea lion cerebrospinal fluid LC-MS/MS

ABSTRACT: We conducted a pilot label-free LC-MS/MS study to profile and compare the cerebral spinal fluid from California sea lions with domoic acid toxicosis and without domoic acid toxicosis. CSF samples from a 8 animals with DAT and 3 controls were run on a nano LC-MS/MS (Triple TOF) and searched against a mammalian database.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Zalophus Californianus (california Sealion)

TISSUE(S): Cerebrospinal Fluid

SUBMITTER: Benjamin Neely

LAB HEAD: Michael G. Janech

PROVIDER: PXD002105 | Pride | 2015-09-21

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	2011-6-17NeelyCSLCSF1.mgf	Mgf
	2011-6-17NeelyCSLCSF1.pride.mgf.gz	Mgf
	2011-6-17NeelyCSLCSF1.wiff	Wiff
	2011-6-17NeelyCSLCSF1.wiff.mtd	Wiff
	2011-6-17NeelyCSLCSF1.wiff.scan	Wiff

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Publications

Proteomic analysis of cerebrospinal fluid in California sea lions (Zalophus californianus) with domoic acid toxicosis identifies proteins associated with neurodegeneration.

Neely Benjamin A BA Soper Jennifer L JL Gulland Frances M D FM Bell P Darwin PD Kindy Mark M Arthur John M JM Janech Michael G MG

Proteomics 20151013 23-24

Proteomic studies including marine mammals are rare, largely due to the lack of fully sequenced genomes. This has hampered the application of these techniques toward biomarker discovery efforts for monitoring of health and disease in these animals. We conducted a pilot label-free LC-MS/MS study to profile and compare the cerebrospinal fluid from California sea lions with domoic acid toxicosis (DAT) and without DAT. Across 11 samples, a total of 206 proteins were identified (FDR<0.1) using a comp ...[more]

PMID: 26364553

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