Proteomics

Dataset Information

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Comparative analysis of mouse muscles types


ABSTRACT: The dataset presents FASP- and LC-MS/MS-based analysis of whole lysates of tree types of mouse muscle tissue: white and red muscle fibers and heart muscle. The measurements were performed using five biological replicates for each tissue type. The dataset cover 7200 proteins. The data were used for calculation of protein titers using the 'total protein method' (TPA) method.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Skeletal Muscle

SUBMITTER: Jacek Wisniewski  

LAB HEAD: Jacek R Wisniewski

PROVIDER: PXD002152 | Pride | 2016-06-21

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
20140930_Qep7_JRW_SA_F47_L2.raw Raw
20140930_Qep7_JRW_SA_F47_L6.raw Raw
20140930_Qep7_JRW_SA_F47_T2.raw Raw
20140930_Qep7_JRW_SA_F47_T6.raw Raw
20140930_Qep7_JRW_SA_F49_L2.raw Raw
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Publications

Proteomics Unveils Fibroblast-Cardiomyocyte Lactate Shuttle and Hexokinase Paradox in Mouse Muscles.

Rakus Dariusz D   Rakus Dariusz D   Gizak Agnieszka A   Wiśniewski Jacek R JR  

Journal of proteome research 20160624 8


Quantitative mapping, given in biochemically interpretable units such as mol per mg of total protein, of tissue-specific proteomes is prerequisite for the analysis of any process in cells. We applied label- and standard-free proteomics to characterize three types of striated muscles: white, red, and cardiac muscle. The analysis presented here uncovers several unexpected and novel features of striated muscles. In addition to differences in protein expression levels, the three muscle types substan  ...[more]

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