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M. truncatula drought response is affected by nodulation

ABSTRACT: Two groups of plants were nodulated with Sinorhizobium medicae or S. meliloti; the third group grew in a rhizobia-free medium and received mineral nitrogen fertilizer. In addition to growth analyses, physiological and molecular responses of the two systems were assessed using ionomic, metabolomic and proteomic techniques.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Medicago Truncatula (barrel Medic) (medicago Tribuloides)

TISSUE(S): Leaf

SUBMITTER: Stefane Wienkoop

LAB HEAD: Stefanie Wienkoop

PROVIDER: PXD002162 | Pride | 2022-02-24

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	N11_NN_R10_C.RAW	Raw
	N11_NN_R10_C.msf	Msf
	N12_NN_R10_C.RAW	Raw
	N12_NN_R10_C.msf	Msf
	N13_NN_R10_C.RAW	Raw

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1 - 5 of 170

Publications

Evidence for a rhizobia-induced drought stress response strategy in Medicago truncatula.

Staudinger Christiana C Mehmeti-Tershani Vlora V Gil-Quintana Erena E Gonzalez Esther M EM Hofhansl Florian F Bachmann Gert G Wienkoop Stefanie S

Journal of proteomics 20160123

Drought stress hampers plant energy and biomass production; however it is still unknown how internal C:N balance and rhizobial symbiosis impact on plant response to water limitation. Here, the effect of differential optimal nitrogen nutrition and root nodule symbiosis on drought stress and rehydration responses of Medicago truncatula was assessed. Two groups of plants were nodulated with Sinorhizobium medicae or Sinorhizobium meliloti--differing in the performance of N fixation; the third group ...[more]

PMID: 26812498

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Medicago truncatula R108 in symbiosis with rhizobia wt or rhizobia mutant for BacA. Comparison of the transcriptomic response of Medicago truncatula plants.

Project description:12plex_medicago_2013-08 - r108 in symbiosis with rhizobia wt or rhizobia mutant for baca. - Two experiments to compare the transcriptomic response of medicago plants: Agar medium versus Phytagel medium (exp1) and rhizobium WT versus BacA (exp2). - Medicago truncatula ecotype R108 was inoculated with the symbiotic rhizobium Sinorhizobium meliloti strain Sm1021 and with its derivative mutant delta bacA. Nodules were collected 13 days after inoculation, and RNA were prepared for transcriptome analysis, there were three biological independant experiements.

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Rhizobial and non/mycorrhizal Medicago truncatula roots and shoots transcriptome

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Overexpression of cyaJ in Rm2011 to study effects of increased cAMP on the transcriptome of Sinorhizobium meliloti

Project description:Alpha-rhizobia are soil-dwelling alphaproteobacteria existing either in free-living states or in symbiosis with a leguminous plant host. cyaJ is a putative adenylate/guanylate cyclase transmembrane protein. Overexpression of cyaJ leads to increased > levels of cAMP. To investigate the effect of increased cAMP on the transcriptome of Sinorhizobium meliloti we performed microarray analysis of > Sinorhizobium meliloti Rm2011 carrying an empty vector pWBT or cyaJ overexpression construct pWBT-cyaJ.

2016-08-17 | E-MTAB-4932 | biostudies-arrayexpress

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Project description:For transcript analysis of responses in Medicago truncatula to its symbiont Sinorhizobium meliloti wild type or the succinoglycan-deficient exoY mutant we compared transcripts from line A17 roots inoculated with 25 mL OD = 0.05 S. meliloti wild type or exoY mutant. Keywords: 1 line; 2 S. meliloti samples

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Expression data of Nodule Cysteine-Rich (NCR) Defensin-Like (DEFL) genes in different stages of nodule development in Medicago truncatula

Project description:We have undertaken a detailed study to identify mechanisms regulating expression of NCRs. We used a custom Affymetrix oligonucleotide microarray to examine the expression changes of 566 NCRs in different stages of nodule development. Additionally, rhizobial mutants were used to understand the importance of the rhizobial components in induction of NCRs. Early NCRs were detected during the initial infection of rhizobia in nodules and continue to be expressed into the late stages of nodule development. Late NCRs were induced concomittant with bacteroid development in the nodules. The induction of these groups of genes was correlated with the number and morphology of rhizobia in the nodule. We used a custom Affymetrix chip containing 684 probe sequences of Medicago DEFLs to explore the expression patterns of NCRs in nodules inoculated with Sinorhizobium meliloti 1021(Sm1021) at marked developmental stages and nodules inoculated with various mutants derived from Sm1021 totalling 14 different treatments. Each treatment was supported by three biological replicates giving a grand total of 42 samples.

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Salt stress enhances early symbiotic gene expression in Medicago truncatula and induces a stress-specific set of rhizobium-responsive genes

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Nodule Zone Specific Proteome - Integrated analysis of zone-specific protein and metabolite profiles within nitrogen-fixing Medicago truncatula-Sinorhizobium medicae nodules

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2017-07-17 | PXD006683 | Pride

The subunit 3 of the SUPERKILLER 3 (SKI3) complex mediates miR172-directed cleavage of Nodule Number Control 1 (NNC1) to modulate nodulation in Medicago truncatula

Project description:Legumes and rhizobia establish a nitrogen-fixing symbiosis that involves the formation of a lateral root organ, the nodule, and the infection process that allows intracellular accommodation of rhizobia within nodule cells. This process involves significant gene expression changes regulated at the transcriptional and post-transcriptional levels. We have previously shown that a transcript encoding the subunit 3 of the Superkiller Complex (SKI), which guides mRNAs to the exosome for 3´-to-5´ degradation, is required for nodule formation and bacterial persistence within the nodule, as well as the induction of early nodulation genes (e.g., MtENOD40) during the Medicago truncatula-Sinorhizobium meliloti symbiosis. Here, we reveal through transcript degradome and small RNA sequencing analysis that knockdown of MtSKI3 impairs the miR172-directed endonucleolytic cleavage of the mRNA encoding Nodule Number Control 1 (MtNNC1), an APETALA2 transcription factor that negatively modulates nodulation. Knockdown of MtNNC1 enhances nodule number, bacterial infection, and the induction of MtENOD40 upon inoculation with S. meliloti whereas overexpression of a miR172-resistant form of MtNNC1 significantly reduces nodule formation. This work identifies miR172 cleavage of MtNNC1 and its control by MtSKI3, a component of the 3´-to- 5´mRNA degradation pathway, as a new regulatory hub controlling indeterminate nodulation.

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Project description:We characterized transcriptomes of a Sinorhizobium meliloti wild type strain (CL150) expressing either Ca. Liberibacter asiaticus ctrA or Sinorhizobium meliloti ctrA

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