Proteomics

Dataset Information

0

Fly Stress - Fasting, but Not Aging, Dramatically Alters the Redox Status of Cysteine Residues on Proteins in Drosophila melanogaster

ABSTRACT: Altering the redox state of cysteine residues on protein surfaces is an important response to environmental challenges. While ageing and fasting alter many redox processes, the role of cysteine residues is uncertain. To address this we used a redox proteomic technique, Oxidative Isotope Coded Affinity TAgs (OxICAT), to assess cysteine residue redox changes in Drosophila melanogaster during ageing and fasting. This approach enabled us to simultaneously identify and quantify the redox state of several hundred cysteine residues in vivo.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Whole Body

SUBMITTER: Andrew James  

LAB HEAD: Michael Patrick Murphy

PROVIDER: PXD002195 | Pride | 2018-10-24

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
CatalaseOverexControl.zip Other
CatalaseOverexH2O2.zip Other
CatalaseOverexPQ.zip Other
CatalaseOverexStarve.zip Other
SupplementalTable1.zip Other
Items per page:
1 - 5 of 15
altmetric image

Publications

Fasting, but Not Aging, Dramatically Alters the Redox Status of Cysteine Residues on Proteins in Drosophila melanogaster.

Menger Katja E KE   James Andrew M AM   Cochemé Helena M HM   Harbour Michael E ME   Chouchani Edward T ET   Ding Shujing S   Fearnley Ian M IM   Partridge Linda L   Murphy Michael P MP  

Cell reports 20150618 12

Altering the redox state of cysteine residues on protein surfaces is an important response to environmental challenges. Although aging and fasting alter many redox processes, the role of cysteine residues is uncertain. To address this, we used a redox proteomic technique, oxidative isotope-coded affinity tags (OxICAT), to assess cysteine-residue redox changes in Drosophila melanogaster during aging and fasting. This approach enabled us to simultaneously identify and quantify the redox state of s  ...[more]

Similar Datasets

Proteomics Effects of the antibacterial compund Allicin from garlic on Escherichia coli
2016-05-02 | PXD003063 | Pride
Proteomics Quantifying changes in the bacterial thiol redox proteome during host-pathogen interaction
2019-01-07 | PXD011386 | Pride
Proteomics Monitoring of protein thiol-oxidation in S.aureus USA300 under hypochlorite stress using OxICAT approach.
2016-12-22 | PXD004918 | Pride
Proteomics Combining redox proteomics with proximity labeling to monitor localized cysteine oxidation
2024-05-21 | PXD034118 | Pride
Proteomics Analysis of ageing-dependent thiol oxidation reveals early oxidation of proteins involved in core proteostasis functions
2024-02-07 | PXD042047 | Pride
Proteomics Proteomic Analyses of Cysteine Redox in High-fat-fed and Fasted Mouse Livers: Implications for Liver Metabolic Homeostasis
2022-03-03 | PXD006612 | Pride
Proteomics Proteome-wide analysis of cysteine oxidation reveals metabolic sensitivity to redox stress, PART 1
2018-03-06 | PXD006363 | Pride
Proteomics OxRAC Analysis of Cysteine Oxidation After EGF stimulation in A431 Cells
2020-01-10 | PXD010880 | Pride
Proteomics Proteome-wide analysis of cysteine oxidation reveals metabolic sensitivity to redox stress, PART 3
2018-03-06 | PXD006373 | Pride
Proteomics Proteome-wide analysis of cysteine oxidation reveals metabolic sensitivity to redox stress, PART 2
2018-03-06 | PXD006372 | Pride