Dataset Information

Mouse liver microsomes phosphorylation

ABSTRACT: The microsomes are re-formed vesicle-like artifacts, not ordinarily present in living cells, from pieces of the endoplasmic reticulum (ER), plasma membrane, mitochondria, and Golgi apparatus of broken eukaryotic cells. Typically, microsomes are separated by differential centrifuged from homogenized of cell or tissue. Based on enrichment of ER fractions, prepared microsomes mainly show a large of metabolic activity following high amount of metabolic enzyme, as cytochrome P450 (CYP). CYPs are important components of xenobiotic-metabolizing monooxygenase accounting for an approximate 75% of biotransformation of clinically relevant drugs. Hepatic CYP are ER-anchored hemoproteins involved in the metabolism of numerous endo- and xenobiotics including drugs, steroids, and carcinogens. The CYPs catalytic activity is subject to regulation via phosphorylation. In several previous studies, 8 CYP phosphorylation sites had been discovered in human. Protein kinase A (PKA) and protein kinase C (PKC) were identified as a major catalyst for the phosphorylation of CYPs in 2003. The phosphorylation of CYPs process to their ubiquitination undergoing ubiquitin-dependent 26S proteosomal degradation.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Liver

SUBMITTER: Oh Kwang Kwon

LAB HEAD: Sangkyu Lee

PROVIDER: PXD002221 | Pride | 2015-08-24

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20140528_KBNU_LSK_pho-MLM_CID-ETD_000_01.raw	Raw
	20140528_KBNU_LSK_pho-MLM_CID-ETD_025_02.raw	Raw
	20140528_KBNU_LSK_pho-MLM_CID-ETD_050_03.raw	Raw
	20140528_KBNU_LSK_pho-MLM_CID-ETD_100_04.raw	Raw
	20140528_KBNU_LSK_pho-MLM_CID-ETD_250_05.raw	Raw

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Publications

In-depth proteomics approach of secretome to identify novel biomarker for sepsis in LPS-stimulated endothelial cells.

Kwon Oh Kwang OK Lee Wonhwa W Kim Sun Ju SJ Lee You-Mie YM Lee Ju Yeon JY Kim Jin Young JY Bae Jong-Sup JS Lee Sangkyu S

Electrophoresis 20150913 23

Sepsis and septic shock, which are conditions triggered by infection, occur with high incidence in emergency departments and are among the most common causes of death in hospitalized patients worldwide. Therefore, the identification of sepsis biomarkers for the rapid diagnosis is a major goal for researchers in the field of critical care. Endothelial cells play a pivotal role in orchestrating the inflammatory response triggered by sepsis. In this study, we used proteomics to investigate the secr ...[more]

PMID: 26257168

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